I'm doing qPCR for both DLD1 and RKO cells (cancer cell lines) and amplified two genes of interest for both cell types.
1. Expected results were obtained for DLD1 cells but for RKO cells, qPCR results didn't show a Cq value for the same two genes. I repeated qPCR for RKO cells starting from making cDNA again and got the same results. Please let me know what could be the possible reasons behind this and what could be done to troubleshoot this issue?
2. Also, please recommend me to troubleshoot getting higher Cq values for housekeeping genes used in qPCR.