Udeshi Wickramarachchi

5 Questions 8 Answers 0 Followers

Questions related from Udeshi Wickramarachchi

How to match the age and weight of mice?

I have two groups of mice representing WT and knockouts. Each mice group (WT and KO) will be allocated to 4 experimental categories. I need to allocate these available mice into these experimental...

14 November 2023 8,543 1 View

Can we reuse the processed samples used in LCMS?

I'm doing a targeted metabolite analysis using LCMS. I did a LCMS assay once using a set of serum samples processed for LCMS. However I wanted to repeat the LCMS run, using the same set of samples...

11 October 2023 9,004 3 View

What are the possible reasons for not getting a Ct/Cq value for the amplification of some genes in qPCR?

I'm doing qPCR for both DLD1 and RKO cells (cancer cell lines) and amplified two genes of interest for both cell types. 1. Expected results were obtained for DLD1 cells but for RKO cells, qPCR...

20 April 2023 8,804 3 View

How to transfer cells cultured in a 100mm dish to a 6-well plate?

I have a cell culture of DLD1 cells and I need to seed them into a 6-well plate for RNA isolation. Please clarify me whether the following procedure would work or any other recommended method to...

13 March 2023 3,428 10 View

Please recommend an efficient method to reduce cell clumping in DLD1 cancer cells?

When I try to culture DLD1 cancer cell line, they always get clumped into small clusters even after doing a decent amount of cell blowing to separate them during seeding and cell passage.

28 February 2023 9,192 7 View