During catalytic reduction study of p-nitrophenol by newly synthesized hybrids, the characteristic sharp peak of PNP (in reference) is split into two peaks after reduction. Is it an indication of possible degradation of PNP?
Hello Madam
1) Reduce the volume from 20 ul to 5 ul and even reduce the concentration of solution.
2) please check any leakage in system or tighten all the ferrules.
Thank you
If all technical parameters are unchanged, then you have modified your compunds.
Your sharp peak of reference is not as sharp but distorted may be due to co-eluting impurity.
Your column is DYING (thus you will see a shoulder and the RTs get longer). Remember, you're injecting other 'crap' on the column every time you make an injection. Maybe you are even 'reducing' your column!
Be sure any excess 'reducing agent' is oxidized/neutralized. In a similar fashion we neutralize the pH of a sample before injection so it does not interact with the column.
- Badges
- Science method