Hi,
I have a simple question to ask!
What are the pitfalls of indirect immunofluorescence studies with polyclonal primary antibody aimed for determination of subcellular localization of a particular protein?
Thanks
Hello
Disadvantages of indirect immunofluorescence include the potential for cross-reactivity and the need to find primary antibodies that are not raised in the same species or of different isotypes when performing multiple-labeling experiments. Also, Samples with endogenous immunoglobulin may exhibit a high background.
The papers in links here discussed this issue
will help you
Good Luck
http://link.springer.com/article/10.1007%2FBF01962186
http://www.dako.com/08002_03aug09_ihc_guidebook_5th_edition_chapter_10.pdf
http://www.ncbi.nlm.nih.gov/pubmed/6383712
http://www.mdbioproducts.com/node/156
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