What are the parameters of stress we can study in Zebrafish embryo after toxicant exposure?

More Archisman Mahapatra's questions See All

How to quantify the reaction intensity in enzyme histochemistry?

I have done enzyme histochemistry for several enzymes in fish gill tissues, now the reviewer wants quantitative data for the same.

03 April 2019 6,959 3 View

Horizontal or Vertical laminar flow, which is best for cell line and RNA/DNA based works?

Which will be best for my application.

07 August 2018 6,035 4 View

How to measure Insulin level in Zebrafish?

How to measure insulin level in Zebrafish tissue homogenate or Serum samples? Will human Insulin ELISA kits work?

06 July 2018 4,996 3 View

Most convenient method for repeated blood collection from Adult Zebrafish?

Don't have the access to any needle puller instrument, to make glass capillary needles from microcapillary tubes. Tried using 1 ml insulin syringe instead, blood collected successfully once, but...

06 July 2018 2,871 0 View

During Zebrafish experimental setup, if I keep 3 fish/Lt of water in each tank is it necessary to use aeration pumps?

I am using semi static system, tank size is of 15 ltr capacity.

06 July 2018 6,954 1 View

How much blood could be collected from an adult Zebrafish?

I need 500 ul blood for my study, how many Fishes should I kept in a group so that I can get required amount of blood.

06 July 2018 9,366 2 View

Why only male Zebrafishes were used in Obesity/diabetes disease studies?

In many of the articles only male fishes were kept in experimental groups. What is the reason? why females are avoided?

06 July 2018 3,403 5 View

Can we get Protein expression profiles along with quantitative gene expression in nCounter® SPRINT Profiler by NanoString?

The nCounter® SPRINT Profiler by Nanostring Technologies is said to be used in DNA, RNA and Protein profiling from the same sample. Will the result be considered if I don't do Western Blot to...

05 June 2018 1,171 4 View

Protocol for single cell suspension of hepatopancreatic tissue of Zebrafish?

Detailed Protocol needed, Thanks in advance.

05 June 2018 575 1 View

Zebrafish ( Tuebingen) dealers within India at cheapest rates?

I need address of dealers who can provide me Zebrafishes (Tuebingen strain) at reasonable prices.

05 June 2018 3,045 0 View

How to quantify polystyrene microplastic (8 micron) bioaccumulation in fish tissue?

"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...

05 August 2024 853 3 View

What is the role of oxidative stress in toxin-induced carcinogenesis?

What is the role of oxidative stress in toxin-induced carcinogenesis? I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from...

02 August 2024 3,174 1 View

Could GSH level be more in CCL4 rats than negative control rats?

i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....

26 July 2024 1,887 0 View

Can we eliminate the stress singularity at the tip of the crack by manipulating the elastic constants?

The aim of the research here is to prevent the propagation of the crack in the fabricated elastic medium with useful applications.

25 July 2024 9,976 3 View

How do nutrient transporters and aquaporins interact under abiotic stress in plants?

"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...

23 July 2024 9,109 0 View

Best method for extracting RNA from morulae?

Best method and number of embryos for extracting RNA from pig morulae?

21 July 2024 4,398 1 View

Can anyone recommend a brand of cas9 protein that can be used for in-vivo editing in model animals?

zebrafish and mouse

19 July 2024 9,158 1 View

How can i generate a CRISPR knockin mutation zebrafish model with a reporter?

Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...

14 July 2024 6,240 0 View

How can alternative crops for augmenting farmers’ income in abiotic stress regions and impact of abiotic environmental stresses on crop quality?

13 July 2024 6,826 5 View

Please give me the scoring system of Academic stress scale developed by Rajendra and Kalikapan?

please give me the scoring system of Academic stress scale developed by Rajendra and Kalikapan?

11 July 2024 2,054 3 View