I have done enzyme histochemistry for several enzymes in fish gill tissues, now the reviewer wants quantitative data for the same.
Article Quantitation of Protein Expression and Co-localization Using...
Article Quantitative comparison of immunohistochemical staining meas...
I hope this article can be useful
Please see these articles:
Article Evaluation of sexual dimorphism of histochemical activity of...
Article HISTOCHEMICAL DOPA OXIDASE ACTIVITY IN LABORATORY RATS DERMA...
Which will be best for my application.
07 August 2018 6,035 4 View
How to measure insulin level in Zebrafish tissue homogenate or Serum samples? Will human Insulin ELISA kits work?
06 July 2018 4,996 3 View
Don't have the access to any needle puller instrument, to make glass capillary needles from microcapillary tubes. Tried using 1 ml insulin syringe instead, blood collected successfully once, but...
06 July 2018 2,871 0 View
I am using semi static system, tank size is of 15 ltr capacity.
06 July 2018 6,954 1 View
I need 500 ul blood for my study, how many Fishes should I kept in a group so that I can get required amount of blood.
06 July 2018 9,366 2 View
In many of the articles only male fishes were kept in experimental groups. What is the reason? why females are avoided?
06 July 2018 3,403 5 View
The nCounter® SPRINT Profiler by Nanostring Technologies is said to be used in DNA, RNA and Protein profiling from the same sample. Will the result be considered if I don't do Western Blot to...
05 June 2018 1,171 4 View
Detailed Protocol needed, Thanks in advance.
05 June 2018 575 1 View
I need address of dealers who can provide me Zebrafishes (Tuebingen strain) at reasonable prices.
05 June 2018 3,045 0 View
After giving exposure of different concentrations of a toxicant to zebrafish embryos, what are the parameters we can study to measure the toxic impact of that particular chemical?
05 June 2018 7,380 3 View
"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...
05 August 2024 853 3 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
First, thanks for taking the time to read my puzzle 🥹! I am currently working on the methodology part of my dissertation, and I have chosen a questionnaire as the instrument for my quantitative...
27 July 2024 3,457 3 View
I have three linkage maps that needs to be integrated to create a consensus map for QTL mapping.
25 July 2024 6,505 2 View
I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...
23 July 2024 8,492 3 View
In the aquaculture and fish nutrition research field, a number of growth and somatic indexes are often used, including specific growth rate (SGR), feed conversion rate (FCR), protein efficiency...
22 July 2024 2,480 4 View
Autonomous Language Learning can be implemented from primary to tertiary education. Practioners and students report on its effectiveness, however, there appears to be little quantitative or...
20 July 2024 2,592 2 View
Chemical Engineering /Food Engineering
20 July 2024 4,152 4 View
Greetings! I am looking for materials on quantitative and qualitative research. What are the methods of collecting information and the principles of their implementation? I would like to make an...
20 July 2024 2,033 7 View