Horizontal or Vertical laminar flow, which is best for cell line and RNA/DNA based works?

Which will be best for my application.

07 August 2018 5,964 4 View

Most convenient method for repeated blood collection from Adult Zebrafish?

Don't have the access to any needle puller instrument, to make glass capillary needles from microcapillary tubes. Tried using 1 ml insulin syringe instead, blood collected successfully once, but...

06 July 2018 2,809 0 View

How to measure Insulin level in Zebrafish?

How to measure insulin level in Zebrafish tissue homogenate or Serum samples? Will human Insulin ELISA kits work?

06 July 2018 4,918 3 View

During Zebrafish experimental setup, if I keep 3 fish/Lt of water in each tank is it necessary to use aeration pumps?

I am using semi static system, tank size is of 15 ltr capacity.

06 July 2018 6,883 1 View

How much blood could be collected from an adult Zebrafish?

I need 500 ul blood for my study, how many Fishes should I kept in a group so that I can get required amount of blood.

06 July 2018 9,300 2 View

Why only male Zebrafishes were used in Obesity/diabetes disease studies?

In many of the articles only male fishes were kept in experimental groups. What is the reason? why females are avoided?

06 July 2018 3,328 5 View

Protocol for single cell suspension of hepatopancreatic tissue of Zebrafish?

Detailed Protocol needed, Thanks in advance.

05 June 2018 503 1 View

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05 June 2018 1,100 4 View

What are the parameters of stress we can study in Zebrafish embryo after toxicant exposure?

After giving exposure of different concentrations of a toxicant to zebrafish embryos, what are the parameters we can study to measure the toxic impact of that particular chemical?

05 June 2018 7,313 3 View

Zebrafish ( Tuebingen) dealers within India at cheapest rates?

I need address of dealers who can provide me Zebrafishes (Tuebingen strain) at reasonable prices.

05 June 2018 2,969 0 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View