How to quantify the reaction intensity in enzyme histochemistry?

I have done enzyme histochemistry for several enzymes in fish gill tissues, now the reviewer wants quantitative data for the same.

03 April 2019 6,835 3 View

Horizontal or Vertical laminar flow, which is best for cell line and RNA/DNA based works?

Which will be best for my application.

07 August 2018 5,964 4 View

Most convenient method for repeated blood collection from Adult Zebrafish?

Don't have the access to any needle puller instrument, to make glass capillary needles from microcapillary tubes. Tried using 1 ml insulin syringe instead, blood collected successfully once, but...

06 July 2018 2,809 0 View

How to measure Insulin level in Zebrafish?

How to measure insulin level in Zebrafish tissue homogenate or Serum samples? Will human Insulin ELISA kits work?

06 July 2018 4,918 3 View

During Zebrafish experimental setup, if I keep 3 fish/Lt of water in each tank is it necessary to use aeration pumps?

I am using semi static system, tank size is of 15 ltr capacity.

06 July 2018 6,883 1 View

How much blood could be collected from an adult Zebrafish?

I need 500 ul blood for my study, how many Fishes should I kept in a group so that I can get required amount of blood.

06 July 2018 9,300 2 View

Why only male Zebrafishes were used in Obesity/diabetes disease studies?

In many of the articles only male fishes were kept in experimental groups. What is the reason? why females are avoided?

06 July 2018 3,328 5 View

No title

05 June 2018 1,100 4 View

What are the parameters of stress we can study in Zebrafish embryo after toxicant exposure?

After giving exposure of different concentrations of a toxicant to zebrafish embryos, what are the parameters we can study to measure the toxic impact of that particular chemical?

05 June 2018 7,313 3 View

Zebrafish ( Tuebingen) dealers within India at cheapest rates?

I need address of dealers who can provide me Zebrafishes (Tuebingen strain) at reasonable prices.

05 June 2018 2,969 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Is RD-ES grow attached or in suspension or both?

We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...

24 February 2021 5,183 1 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View