How to quantify the reaction intensity in enzyme histochemistry?

I have done enzyme histochemistry for several enzymes in fish gill tissues, now the reviewer wants quantitative data for the same.

03 April 2019 6,835 3 View

Horizontal or Vertical laminar flow, which is best for cell line and RNA/DNA based works?

Which will be best for my application.

07 August 2018 5,964 4 View

Most convenient method for repeated blood collection from Adult Zebrafish?

Don't have the access to any needle puller instrument, to make glass capillary needles from microcapillary tubes. Tried using 1 ml insulin syringe instead, blood collected successfully once, but...

06 July 2018 2,809 0 View

How to measure Insulin level in Zebrafish?

How to measure insulin level in Zebrafish tissue homogenate or Serum samples? Will human Insulin ELISA kits work?

06 July 2018 4,918 3 View

During Zebrafish experimental setup, if I keep 3 fish/Lt of water in each tank is it necessary to use aeration pumps?

I am using semi static system, tank size is of 15 ltr capacity.

06 July 2018 6,883 1 View

Why only male Zebrafishes were used in Obesity/diabetes disease studies?

In many of the articles only male fishes were kept in experimental groups. What is the reason? why females are avoided?

06 July 2018 3,328 5 View

Protocol for single cell suspension of hepatopancreatic tissue of Zebrafish?

Detailed Protocol needed, Thanks in advance.

05 June 2018 503 1 View

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05 June 2018 1,100 4 View

What are the parameters of stress we can study in Zebrafish embryo after toxicant exposure?

After giving exposure of different concentrations of a toxicant to zebrafish embryos, what are the parameters we can study to measure the toxic impact of that particular chemical?

05 June 2018 7,313 3 View

Zebrafish ( Tuebingen) dealers within India at cheapest rates?

I need address of dealers who can provide me Zebrafishes (Tuebingen strain) at reasonable prices.

05 June 2018 2,969 0 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View

Which polarizers are usually used to measure the birefringence of zebrafish muscles?

I already found several publications on that topic, however, the specifications for the used filters are often missing. Some more specific information than "fish is placed between to polarizing...

23 February 2021 1,934 1 View

Measuring Total immunoglobulin whole-body of fish larvae?

Hi Dear All, I prepared the extract of fish larvae whole body with PBS. Then protein concentration of the whole-body extraction samples determined by the Folin-Ceiocalteu-Phenol method (Lowry...

17 February 2021 1,971 3 View

What is the identity of additional bands found during fish gut microbiome 16S V4 PCR amplification?

Hi, I am carrying out test PCRs for 16S amplicon sequencing on Illumina MiSeq. I am using standard V4 primers: 515F/806R. The aim is to profile the microbiome of fish guts under different...

16 February 2021 5,668 6 View

Is there any free software for behaviour analysis of zebrafish ?

I need to do novel tank test and light/dark test for zebrafish. So I need a tracking software for tracking in these tests. Is there any free softwares available for tracking of zebrafish ?

16 February 2021 1,541 1 View

Is this the fragment of a sauropod tooth?

Dear colleagues. Fragments of this species are very rare from a terrestrial Lower Cretaceous locality from Germany. Up to now from this locality sauropods could not be proved with certainty. Other...

14 February 2021 2,310 1 View