Archisman Mahapatra

14 Questions 15 Answers 0 Followers

Questions related from Archisman Mahapatra

How to quantify the reaction intensity in enzyme histochemistry?

I have done enzyme histochemistry for several enzymes in fish gill tissues, now the reviewer wants quantitative data for the same.

04 April 2019 6,912 3 View

Horizontal or Vertical laminar flow, which is best for cell line and RNA/DNA based works?

Which will be best for my application.

08 August 2018 6,006 4 View

How to measure Insulin level in Zebrafish?

How to measure insulin level in Zebrafish tissue homogenate or Serum samples? Will human Insulin ELISA kits work?

07 July 2018 4,967 3 View

Most convenient method for repeated blood collection from Adult Zebrafish?

Don't have the access to any needle puller instrument, to make glass capillary needles from microcapillary tubes. Tried using 1 ml insulin syringe instead, blood collected successfully once, but...

07 July 2018 2,848 0 View

Why only male Zebrafishes were used in Obesity/diabetes disease studies?

In many of the articles only male fishes were kept in experimental groups. What is the reason? why females are avoided?

07 July 2018 3,370 5 View

How much blood could be collected from an adult Zebrafish?

I need 500 ul blood for my study, how many Fishes should I kept in a group so that I can get required amount of blood.

07 July 2018 9,339 2 View

During Zebrafish experimental setup, if I keep 3 fish/Lt of water in each tank is it necessary to use aeration pumps?

I am using semi static system, tank size is of 15 ltr capacity.

07 July 2018 6,922 1 View

Can we get Protein expression profiles along with quantitative gene expression in nCounter® SPRINT Profiler by NanoString?

The nCounter® SPRINT Profiler by Nanostring Technologies is said to be used in DNA, RNA and Protein profiling from the same sample. Will the result be considered if I don't do Western Blot to...

06 June 2018 1,139 4 View

Zebrafish ( Tuebingen) dealers within India at cheapest rates?

I need address of dealers who can provide me Zebrafishes (Tuebingen strain) at reasonable prices.

06 June 2018 3,010 0 View

What are the parameters of stress we can study in Zebrafish embryo after toxicant exposure?

After giving exposure of different concentrations of a toxicant to zebrafish embryos, what are the parameters we can study to measure the toxic impact of that particular chemical?

06 June 2018 7,354 3 View

Need a protocol for SOD, Catalase and LPO enzyme assay in 96 well plate?

I need detailed protocol. Thanks in advance.

06 June 2018 7,783 4 View

Protocol for single cell suspension of hepatopancreatic tissue of Zebrafish?

Detailed Protocol needed, Thanks in advance.

06 June 2018 544 1 View

What are the methods to follow if I want to study cross talk between molecular pathways?

If I want to study the cross talks between pathways involving more than 20-30 genes, what are the methods I should follow? qRT PCR and then validation by Western Blot or Immunohistochemistry is...

06 June 2018 6,695 2 View

Extraction method for Persistent Organic Pollutants from water and sediment sample for GC-MS analysis?

I need detailed protocol.

05 May 2018 1,770 5 View