What are the other tests which can measure transfection efficiency using nanoparticles without the involvement of cells? Preferably cheap. ?

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Karstedts Catalyst - How Does it Work In an Addition-Cure Silicone Crosslinking Reaction?

Or, really, one of the many other organometallic catalysts that can get this done. From what I've seen, there is no clear answer yet. Perhaps something to do with d-block electron activity but...

28 April 2024 6,666 1 View

How does co-infection with hepatitis B virus (HBV) affect the diagnosis of HDV infection?

In relation to an article titled "Diagnostic Efficacy of Serological Antibody Detection Tests for Hepatitis Delta Virus: A Systematic Review and Meta-Analysis" by Zhenzhen Pan et al. (2023)

08 April 2024 4,432 1 View

How to freeze-dry gold nanoparticles bioconjugated with antibodies?

Some articles about the freeze-drying of nanomaterials, more specifically gold nanoparticles. I would be grateful.

30 November 2023 6,306 2 View

Can anyone suggest any interview questionnaire for leadership style?

Hello! I am currently conducting a qualitative study about leadership styles where I need to determine the leadership style used by my participants and I need help in looking for an already...

22 June 2023 7,753 3 View

How to prepare a stock solution from Ruthenium red powder?

I want to screen strains of lactic acid bacteria (LAB) for the production of exopolysaccharides (EPS) using Ruthenium red milk agar. I already tried to make a stock solution of Ruthenium red...

03 May 2023 4,429 0 View

TCID50 Protocol - 100uL or 200uL?

Hey, so I am writing a TCID50 protocol, and was curious as to why you want to add 200uL of total volume to the 96 well plates - is it just so the cells don't starve? I can't imagine another...

24 January 2023 217 0 View

Where to purchase viruses other than ATCC?

I have been looking to expand our virology department here for a year since joining my current institution. I have only ever purchased viruses from Carolina Biosciences (bacteriophage) and ATCC...

12 January 2023 4,248 2 View

How to purify my RNA samples from salt contamination?

Dear all, A lot of my samples are contaminated with salt after a RNA extraction using the QIAGEN minikit. Can I clean my samples? If yes, what is the protocol? My samples come from various...

17 August 2022 7,627 0 View

Splitting cells from T75 to T175 1:20 dilution ?

Hi there, I need to split cells from a T75 flask into three T175 flasks at a 1:20 dilution. How much volume of cells from the T75 (after I have lifted + washed/EDTA and resuspended in 4ml...

04 July 2022 2,211 1 View

Which statistical test to compare means for fire analysis?

I'm trying to undertake a statistical analysis of some mean proportions of area burnt (Total area burnt vs. areas burnt at various smaller sites within the total area (including an inner and outer...

20 April 2022 8,575 2 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

Binder for ZnO nanoparticles in graphite paste electrode?

I have been using paraffin, but the deposited ZnO still detach from electrode. What is the best binder to modify graphite paste electrode with ZnO nanoparticles?

03 August 2024 4,624 3 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Mossbauer spectroscopy Co0.6Mn0.4Fe2O4 MNPs?

Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.

31 July 2024 9,140 2 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

29 July 2024 3,229 1 View