How to determine the significance of "diagonal" contrasts in two-way ANOVA interactions on SPSS?

Hi all, I'm running a 2 x 2 between-subjects ANOVA with the setup in the attached image (kindly refer to JPG attachment). I understand that calculating simple effects involves determining...

09 February 2021 547 3 View

How to do investigator triangulation in qualitative research?

I am doing a qualitative research with interview as primary data and using thematic analysis. This is my first time doing investigator triangulation. I plan to code the transcriptions...

01 November 2020 4,274 3 View

Are there any alternatives to trasnfection efficiency?

We'd like to test our nanoparticles for transfection efficiency, however, due to COVID 19, limitations hinder us from doing so. Are there any cheap alternatives? Preferrably in plants or other...

06 September 2020 6,398 3 View

What bacterial cells can I use (preferably cheaper with good transfection efficiency) aside from DH5 Alpha cells from E. coli for gene delivery?

I am planning on conducting a research on gene delivery, however , we only have a limited amount of sources, can you suggest bacterial cells that I can use (preferably cheaper with good...

28 August 2019 5,667 3 View

What exactly is the difference between using 95% ethanol and methanol in making a Bradford reagent?And is the filtering process necessary?

I've seen 95% ethanol and methanol being mentioned in protocols and I was wondering whether using one over the other have any drastic affects on the reagent (ie longevity, quality, color...

14 August 2019 292 3 View

What strain of mice should I use for a study in Toxoplasma cyst reduction?

19 July 2017 5,407 1 View

Recommendations for a catalase kit to use on coral?

Does anyone have any recommendations on which catalase kit is best to use on coral tissue? I have narrowed it down to three options: Thermo fisher: Amplex Red Catalase Assay Kit (400...

26 April 2017 8,926 2 View

Is there a questionnaire for teachers to assess the use of the five basic elements of cooperative learning?

I'm looking for a questionnaire for teachers to assess the use of the five basic elements of cooperative learning in the classroom (elementary school). It's for my masterthesis. Thankyou!

19 January 2017 5,932 4 View

Can Anyone Advise on Running Omnibus Test of Group Differences in SmartPLS?

Read 4 answers by scientists to the question asked by Gabrielle Daniels-Gombert on Jul 12, 2016

11 July 2016 5,941 3 View

Which applications do you consider important for the health area?

Which applications do you consider important for the health area?

01 January 1970 3,273 11 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How can I calculate the rdf of water molecules around 'Na' ions that are closest(adsorbed) to the nanoparticle(solute)?

Hi, I want to calculate the radial distribution function for water molecules around the Na+ ions adsorbed on the surface of a spherical nanoparticle using the gromacs trajectory dynamically? For...

28 February 2021 5,650 1 View