Hey, so I am writing a TCID50 protocol, and was curious as to why you want to add 200uL of total volume to the 96 well plates - is it just so the cells don't starve? I can't imagine another reason, but I remember having dumped or aspirating media, adding 100uL of infectious media - or sample - to the wells, incubating for an hour and adding warmed 100uL of 2%FBS 1%PSG EMEM or DMEM depending on cell line.

What is the reasoning?

Thank you!

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