I am currently doing Kupffer Cell primary cultures (by differential centrifugation). What are the optimal conditions to culture them? I currently use standard culture-treated 24 well plates and DMEM high glucose with 10% FBS and 2% pen/strep.
Hey Maite,
. Kupffer cells from liver , not easy .
When we have cultured Kupfer cells, we used currently RPMI medium with 10% FBS and 1% Pen/strep.
After 48h TNF quantification from supernatant.
So, i think , Kupffer cells by the fact that there are Macrophage Like's cells don't need special medium.
But what do you want to do exactly ?
Thank you! Yes, KC's can give you a headache sometimes! I've been successfully isolating them but I want to make the procedure more efficient and reproducible. Did you use special treated or coated plates or did you find that the standard culture-treated ones work just fine??
I use standart culture-treated plates ,macrophages attached rapidly, strongly to plate and non-adherent cells can be remove( other hepatic'cells like endothelial )by washing.
With coated plate , i think that you risk to lost purity and perhaps coating matrix action on KC's. But i have never tired.
Good Luck
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