Can a sample positive for E. canis by smear test, be negative by PCR test?

We received three Dog blood samples for E. canis PCR test. The DNA was extracted using QIAamp DNA blood mini kit and sujected to PCR. The template DNA was subjected to two different PCR using...

11 December 2019 6,801 7 View

Any suggestion for low cost Real-Time PCR?

I am planning to buy a real time PCR instrument for my laboratory in Chennai, India. Budget is my primary concern and I am in a great dilemma to decide which machine might be the best choice for...

07 August 2019 8,015 3 View

Discrepency between nanodrop reading and agarose gel electrophoresis interpretation?

06 July 2019 1,294 10 View

What can be the possible reason for the signal observed in NTC during TaqMan probe based qPCR (Real Time PCR) assay?

Hello everyone, I am carrying out TaqMan probe based Real-Time PCR assay. I am able to observe signal peak for the NTC sample. However upon agarose electrophoresis I was unable to see any band...

06 July 2019 1,392 7 View

Which TaqMan dye combination is best suited for SNP genotyping?

I am planning to standardize a TaqMan duplex PCR assay for allelic discrimination. I intend to use FAM and TAMRA for one probe. Which dye and quencher would be best for the second probe that can...

03 April 2019 5,418 3 View

Why fungal DNA does not yield amplification against ITS-1 and ITS-4 primers?

I tried extracting DNA from an unknown environmental fungal isolate using Qiagen DNeasy extraction kit. However, I was unable to extract DNA in the first attempt. Then I tried increasing the...

02 March 2019 4,917 13 View

What could be the reason for non specific amplification of gDNA upon serial dilution?

The aim of my study was to evaluate the sensitivity the PCR assay for the in-house primers that I had designed. The concentration of the extracted DNA was 564.4 ng/uL with A260/A280 ratio of 1.97...

01 February 2019 185 2 View

Is it possible to differentiate plant tissues based on bisulfite sequencing methodology?

My objective is to identify the tissue of origin used for the plant extracts preparation. Is it possible to extract DNA from any provided plant extracts and subject it to bisulfite sequencing and...

01 February 2019 210 3 View

How upload a sequence with novel mutation in GenBank?

04 May 2016 4,605 6 View

Muller-Hinton agar contains ferrous or ferric ion?

I want to know the oxidation state of the iron ion present in the Muller-Hinton agar. Whether it is ferrous ion or ferric ion?

04 May 2016 1,886 2 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

Please suggest the website or link for the collection of data for (Cancer Incidance Rates in India)?

We are doing research for the prediction of cancer incidance rates increases in India upto next five years. Please help us to get the exact data for Cancer Incidance Rates in India. This will...

19 February 2021 8,478 2 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Access to supercomputer resources in India?

Hello everyone, I want to perform some gaussian calculations and want to use Materials studio for my research work. Can anyone guide me how to get access to supercomputer resources in India for...

15 February 2021 8,264 3 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View