How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

Does anyone know why I would have unexplained emission overlap for flow cytometry?

Hello, I am new to flow cytometry, and was just using a sample cell population to train with the technician. The cells were PAC-1 cells split into four groups. Unstained, transiently transfected...

28 January 2021 969 2 View

Does anyone know of a library for R that includes phi?

I'm looking to produce some phi correlations and I'd rather not do it manually, but I can't find a library for R that includes phi.

18 January 2021 2,808 9 View

In SEM, how to interpret standardised regression weights and their significance?

I have a SEM model that I'm having difficulty interpreting. It has one direct effect with a Beta/standardised regression weight that appears to be high (0.80) and significant (p

31 December 2020 7,604 6 View

Does anyone know how to add custom text to a violin plot in r, ggpubr made using the ggviolin line?

I've produced a violin plot for a point-biserial correlation. Main title is there, x and y are labelled, but I want to add a custom text box with the correlation coefficient and significance...

26 December 2020 4,480 4 View

How do I interpret these correlations and p-values?

I've just run a series of point biserial correlation tests in R between whether or not characters were assigned national identities, and attributions given to their behaviours - results shown in...

17 December 2020 9,825 4 View

Is it possible to fuse Deep Learning Net and Clustering Algotrithm?

What I did (unpublished yet) is extract several (e.g. 16) mask features within Mask R-CNN from multiple images, construct a distance metric to compare them to predict the image class. Each set of...

13 December 2020 9,504 2 View

Are there models trained+evaluated on multiple datasets?

Generalization of COVID-19 models is a very serious challenge. I'm trying to find a model (ct scans or x-rays) that was trained and evaluated on multiple datasets. It would be particular...

03 December 2020 8,863 4 View

Are there models trained on both x-ray and ct scans for COVID-19 prediction?

I've only come across models that were trained and evaluated on one of these data. I don't see why it would be a problem to do both, as there are many similar features.

03 December 2020 8,310 3 View

When examining a series with different numbers of episodes per series, should I treat it as single sample, use harmonic mean or some other approach?

I'm currently examining how the behaviours of fictional characters are explained to child audiences through attribution theory as part of a study into the psychology of how children learn about...

27 November 2020 8,229 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View

What are the possible effects of COVID -19 Vaccine/s on the persons already recovered from the disease?

Persons recovered from COVID -19 are having antibodies against the virus inside their bodies. They are having T cells already sensitized to the antigens of the virus. If they are vaccinated (may...

12 February 2021 4,106 22 View

What is the reason, we got both test and control band on LFA in negative sample?

I am working on Lateral flow assay for virus detection by carbon conjugated polyclonal antibody. We got a high intense band in both test and control line on LFA in negative sample used as buffer....

11 February 2021 6,558 2 View