Ranjith Pathirana

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Questions related from Ranjith Pathirana

How can I maintain the embryogenic potential of a cell culture long-term?

Why does my embryogenic cell culture losing its embryogenic potential after several subcultures? I try to maintain it growing fast by frequent subculture, selecting more dispersed cells,...

22 November 2014 10,095 2 View

What are the reasons for the difficulties in achieving the same cell density in an embryogenic cell culture of the same genotype when repeated?

When I first started a cell culture from leaf explants, it produced a very high density dispersed cell culture and I could produce large amounts of somatic embryos. When I tried to repeat it, the...

21 November 2014 9,391 6 View

How do I test pollen fertility in interspecific and intergeneric hybrids?

Dear All, We have acetocarmine and other staining methods, germination by hanging drop method and we can even stain the germinating pollen on stigma and visualise with aniline blue staining to...

03 November 2014 5,809 7 View

Can viruses spread in tissue culture media from one explant to another?

For virus elimination, we use meristem culture, thermotherapy and cryotherapy in addition to other techniques such as using antiviral compounds. I am sure many of us have several explants in a...

16 October 2014 3,971 9 View

Do you think it is possible to produce a periclinal chimera using two cultivars of the same species or related species?

Hi, Are you aware of chimeras with different ploidy levels in different layers and those having distinct genetic differences for many characters? I am aware of the Pinot series in grape and some...

03 September 2014 3,388 6 View