How to group wells data from growthcurver package?

Hi, I've been using growthcurver package for growth curves experiment. I have the output data per well- but now I want to group wells together according to my meta-data (for instance three...

21 June 2019 6,635 1 View

Failed expression of recombinant nucleation peptide, due to a short spacer between T7 promoter to RBS?

hi all, i'm having a problem to express my gene in E.Coli (BL21) of a nucleation peptide. nowadays i'm suspecting that it might be because of a too short spacer in my vector: (the "map", and the...

27 June 2018 3,214 5 View

Why I get no amplification using degenerative PCR?

Hello, I'm trying to amplify a gene in both eDNA and bacterial DNA (a strain of Bacillus, that should be the positive control) using degenerative primers, their sequence was published in a...

10 December 2017 503 3 View

Purifying 1000 cells from 1.5ml PBS, any ideas?

Hi all, I'm looking for ideas to purify 1000 cells from 1.5ml PBS while minimizing cell loss and as clean as possible (not using beads for example). Also standard centrifugation is not an option...

17 July 2016 2,548 3 View

Can anyone recommend culture conditions for human blood primary dendritic cells?

medium composition, density, anything alse?

12 January 2016 8,909 2 View

Any recommendations for a good antibody against BDNF?

Hi I'm looking for a good antibody anti BDNF to use in Western blot and IHC to characterize BDNF in the mouse brain. Preferably, an antibody that works good for both applications would be...

01 November 2015 3,347 6 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View