How to genotype Cre-mediated recombination events in ROSA26-pCAG-LSL KI mice?

Hello, I am using a mice line which is a cross product of Sun1-tagged mice (Jax #021039) with VIP-Cre mice (Jax # 010908). I'm trying to establish a PCR genotyping protocol to detect whether an...

20 April 2020 7,692 3 View

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Please click the link to take part in the short survey (less than 3 minutes) on the updated EMA Guideline on first-in-human clinical trials. Your data will be collected anonymously....

02 March 2018 7,673 0 View

Hi, Is someone familiar with the DREADD system and the technique of Clozapine administration via drinking water or osmotic mini-pump's in mice model ?

Thanks

08 March 2017 2,988 1 View

Can I use a linearized plasmid that has been stored (in water) at minus 20 for two weeks?

Hello, I have a linearized plasmid (digested and cleaned by phenol/chloroform precipitation) that has been stored in water at minus 20 deg for two weeks. The plasmid is intended to be used as...

27 February 2017 5,388 4 View

How can I suspend nano-powder of Nb in organic solvent?

09 January 2017 1,005 2 View

POWER: For a longitudinal developmental study with 3 time-points, in order to discriminate between trajectories, what is the minimal sample size?

The study is designed to measure executive function in children in 3 consecutive years. The children will come into the lab annually with their parent. Since executive function is itself a highly...

04 October 2015 7,297 3 View

Which are the best tests to assess behavioural consistency in two different populations of fish (grouped by sex, spawning success)?

I performed different coping style tests on two fish populations (grouped by sex and spawning success). Which tests are the most suitable to demonstrate the consistency of individuals (eg. I want...

30 June 2015 6,671 3 View

Can scale score be calculated after more than 25% of items were omitted due to low reliability?

Hi, I'm using a self-reported and validated 9-item questionnaire. Items are presented in Lykert type format (1-5). I calculated Cronbach's alpha (after reverse coding the relevant items) which...

01 January 1970 8,922 2 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to figure out easily the right journal with the right scope for your paper?

According to my experience most of the selected journals during paper submission are based on journal text matching suggested results from Springer, Elsevier,,,Etc. then the manuscript happened to...

01 March 2021 1,786 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View