Which is a good positive control probe for CISH on FFPE mice tissues?
Hello. I am a master student in a Vascular signaling laboratory. I am researching the use of lactone molecules and the effect of PLGA Nanoparticles (NP) with EPA-L on (Human umbilical vein...
06 June 2023 9,879 0 View
tl;dr: I'm looking for studies that used an interview with AI as a research method. Preferably in social sciences, and even better if it is a study that discusses representation or framing theory...
24 March 2023 5,703 4 View
Hello, We are interested in using HCR instead of RNA Scope for single molecule fluorescence in situ hybridization (smFISH). Can someone point out the major pros & cons of each method? Also, does...
05 April 2021 5,021 0 View
I am searching for information, pictures and documentation of built elements which are used in Thai homegardens (especially in Isan). These can be: trellises, shading structures, built seedbeds,...
17 February 2021 6,483 0 View
Hello, I am using a mice line which is a cross product of Sun1-tagged mice (Jax #021039) with VIP-Cre mice (Jax # 010908). I'm trying to establish a PCR genotyping protocol to detect whether an...
20 April 2020 7,823 3 View
Please click the link to take part in the short survey (less than 3 minutes) on the updated EMA Guideline on first-in-human clinical trials. Your data will be collected anonymously....
02 March 2018 7,795 0 View
Hello, I have a linearized plasmid (digested and cleaned by phenol/chloroform precipitation) that has been stored in water at minus 20 deg for two weeks. The plasmid is intended to be used as...
27 February 2017 5,506 4 View
The study is designed to measure executive function in children in 3 consecutive years. The children will come into the lab annually with their parent. Since executive function is itself a highly...
04 October 2015 7,418 3 View
I performed different coping style tests on two fish populations (grouped by sex and spawning success). Which tests are the most suitable to demonstrate the consistency of individuals (eg. I want...
30 June 2015 6,834 3 View
I teach behavioral genetics to undergraduate psychology students and I was struck by the genetic findings for SLI. They seem more specific and well-replicated than for many other phenotypes; for...
18 May 2015 6,579 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...
01 August 2024 1,168 4 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
Hello, I'm trying to measure the conductivity of semiconductor films but since I don't have a commercial four point probe set up I would like to build one on my own in my lab. I have generators,...
30 July 2024 906 2 View
