How to genotype Cre-mediated recombination events in ROSA26-pCAG-LSL KI mice?

Hello, I am using a mice line which is a cross product of Sun1-tagged mice (Jax #021039) with VIP-Cre mice (Jax # 010908). I'm trying to establish a PCR genotyping protocol to detect whether an...

20 April 2020 7,692 3 View

Hi, Is someone familiar with the DREADD system and the technique of Clozapine administration via drinking water or osmotic mini-pump's in mice model ?

Thanks

08 March 2017 2,988 1 View

How can I suspend nano-powder of Nb in organic solvent?

09 January 2017 1,005 2 View

Can scale score be calculated after more than 25% of items were omitted due to low reliability?

Hi, I'm using a self-reported and validated 9-item questionnaire. Items are presented in Lykert type format (1-5). I calculated Cronbach's alpha (after reverse coding the relevant items) which...

01 January 1970 8,922 2 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Is this grounds for theophylline toxicity even though her plasma is within the therapeutic range? if it is, how is that possible?

A patient was given 300 mg Theophylline taken twice a day for the treatment of her bronchial asthma and has a positive clinical response. She has been compliant in taking her medication however,...

09 February 2021 3,489 3 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

How can we increase awareness about Covid 19 prevention in our city among the local population?

The general public must be made aware of the mode of transmission, presenting symptoms and the measures that can be undertaken to prevent the spread of infection. Few options- Media, Webinars...

03 January 2021 3,538 13 View

Are the receptor proteins on the surface of infected cells will increase or decrease the susceptibility of antivirals?

Are the receptor proteins on the surface of infected cells will increase or decrease the susceptibility of antivirals?

22 December 2020 3,242 4 View

How to convert Terapeutic Human Doses to Cell Experimentation Doses?

"Toxicological evaluations revealed that Cur is found to be pharmacologically safe, even up to 12 g per day, as reported by several animal studies and in phase-I clinical trials Similarly,...

07 December 2020 3,012 1 View

How to increase presynaptic neurotransmitter release artificially?

I would like to pharmacologically increase presynaptic neurotransmitter release in mice CNS in vivo (through the extracellular micro-infusion of a compound). Which strategy would you recommand?...

02 November 2020 5,645 3 View

No title

30 October 2020 961 3 View

Poly ICLC or Poly IC structure for docking ?

I want to do docking of Poly ICLC with protein. But, unfortunately there is no crystal structure for Poly ICLC or poly IC. Poly ICLC is a complex of Poly IC, poly lysine, and...

24 September 2020 6,107 1 View

Journals accepting book reviews in Pharmacy field (Pharmacology)?

I had published books as a part of my research interest related to pharmacy field (Pharmacology). Now, I am interested to submit books to review in academic journals (Book review publish). Can any...

19 September 2020 1,666 2 View