How to genotype Cre-mediated recombination events in ROSA26-pCAG-LSL KI mice?

Hello, I am using a mice line which is a cross product of Sun1-tagged mice (Jax #021039) with VIP-Cre mice (Jax # 010908). I'm trying to establish a PCR genotyping protocol to detect whether an...

20 April 2020 7,692 3 View

Can I use a linearized plasmid that has been stored (in water) at minus 20 for two weeks?

Hello, I have a linearized plasmid (digested and cleaned by phenol/chloroform precipitation) that has been stored in water at minus 20 deg for two weeks. The plasmid is intended to be used as...

27 February 2017 5,388 4 View

POWER: For a longitudinal developmental study with 3 time-points, in order to discriminate between trajectories, what is the minimal sample size?

The study is designed to measure executive function in children in 3 consecutive years. The children will come into the lab annually with their parent. Since executive function is itself a highly...

04 October 2015 7,297 3 View

Which are the best tests to assess behavioural consistency in two different populations of fish (grouped by sex, spawning success)?

I performed different coping style tests on two fish populations (grouped by sex and spawning success). Which tests are the most suitable to demonstrate the consistency of individuals (eg. I want...

30 June 2015 6,671 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

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02 March 2021 3,060 3 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View