Good Day.
I am patching primary cortical neurons. I am just curious if you can predict based only on bright-field microscopy and neuron morphology, what neuron types are present in primary cortical neurons?
Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?
05 August 2024 2,333 2 View
Because I have realized that the world tends more and more to do open and free science and there is a trend more and more to choose free databases, free tools and open access platforms.
01 August 2024 10,046 1 View
Hello, I'm planning a shell exchanging experiment with two marine, hermit crab species inside of a tank. I only have one tank available for this experiment. I plan on running 30-40 shell...
20 July 2024 5,676 3 View
hi, I am doing a risk analysis of a cylindrical fuselage with windows. I have applied contact interactions between the different parts, and I have applied a unit value load to a reference point,...
20 July 2024 647 2 View
Hello everyone, I'm currently working on calculating the relative levels of mtDNA using qPCR by comparing the Ct values of a mitochondrial gene (nd1) and a nuclear gene. According to my...
16 July 2024 5,508 0 View
Due to the shifting ocean currents, the planet could be thrown off balance, altering the angle of rotation, and even causing the axis to shift, plunging us into chaos. In reality, it doesn't take...
15 July 2024 8,949 1 View
I have aquired some mites which have been isolated from feeding trays at a black soldier fly farm. These mesostigmatans are not my primary field of interest, although they are very beautiful. Can...
13 July 2024 10,036 5 View
Hello everyone, I am currently working on a project involving spatial transcriptomics on E14 mouse embryos. We need to perform cryosectioning without fixing the embryos. Here is the protocol we...
10 July 2024 5,985 0 View
I am looking for a CRISPR protocol for application in leishmania strains
08 July 2024 9,732 1 View
What do you think is the main advantage of cell membrane-camouflaged nanoparticles, according to your experience?
04 July 2024 6,902 2 View
Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...
07 August 2024 648 0 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
Firing in GABAergic neurons has been characterized in several areas of the brain, but patterns are the same?
04 August 2024 4,090 0 View
Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...
23 July 2024 6,783 1 View
Kindly confirm how to distinguish glia cells and neurons using cresyl violet staining. Also, using cresyl violet nissl staining how to identify the apoptotic neurons ? Does this stain stains...
18 July 2024 2,420 0 View
While living in Boston, which has a fine metro system, there were times that I would exit from underground at the wrong location, thereby expecting a particular visual scene only to be momentarily...
17 July 2024 3,237 10 View
Unlike humans, it is believed that birds do not have a symbolic language system that can be reduced to words (Berwick et al. 2012). But they do have a nervous system that can generate sequences...
14 July 2024 9,852 0 View
Hello everyone, We are trying to differentiate the KOLF-NGN2 hiPSC into neurons - A reference human induced pluripotent stem cell line for lar... . The differentiation is characterized by 2...
09 July 2024 4,002 1 View
Hi all, I am looking glass cilinders that have an open flat bottom and top. Preferably with the inner diameter in the range of 2 cm. It's similar to the ones found on multi-electrode arrays for...
06 July 2024 5,846 1 View
Hi all, I am working on both primary hippocampal mouse neurons and i3Neurons to isolate axonal material. I plate them in microfluidic devices in house made (like any other you can find on the...
01 July 2024 8,070 0 View