17 Questions 19 Answers 0 Followers
Questions related from Simon Miguel Lopez
Hello Everyone I am using 0.25% tryspin to digest my cells for passage/seeding. However, I just notice that the cell quality is not good based on appearance (e.g. many rough cells and clusters...
23 May 2023 2,911 3 View
Good Day. I am doing patch clamp recordings on neurons and HEK cells. I would like to know what are the recommended sampling rates for neurons and HEK cells. I am concerned that my high sampling...
09 May 2023 4,929 3 View
Hell Everyone I am finding out a convincing reason by asking for your thoughts and opinions. I noticed there are are several cre-lines that can be used to express your protein in a brain region...
16 April 2023 1,883 1 View
Good Day Recently in my AP recordings, I often observe that there is a time delay in my current injection recordings. For example, if I set the protocol to inject at 1s, the actual recording...
19 March 2023 2,274 0 View
Good Day. I am patching primary cortical neurons. I am just curious if you can predict based only on bright-field microscopy and neuron morphology, what neuron types are present in primary...
11 March 2023 547 0 View
Good Day. I am just curious if there is any reference that shows what age of primary cortical neurons have a membrane potential range from -60 to -70 mV? Right now, the membrane potential of my...
11 March 2023 5,064 0 View
Good Day Based from your experience, I would like to ask if it is usual that there are dead and floating transfected HEK293 cells after seeding in PLK-coated coverslip? In my experiments, I used...
30 January 2023 2,010 1 View
Hello everyone. Recently, I observe that my HEK293 cells in the 6 well plate have circular cells that are floating in the medium. This is only a small fraction of the whole cell maybe
28 January 2023 8,702 2 View
Hello. I am just curious if there will be changes in function/behaviour if you optogenetically activate a particular region of the neuron (e.g. dendrite), compared to activating the whole neuron...
05 November 2022 6,219 2 View
Hello I just want to ask if you can also recommend research or review articles highlighting the unique receptors found on CD4+ T cells that are not found on other immune cells? Thank you
26 October 2021 2,349 1 View
Hello I just want to gather insights on how much time does your reaction take when cleaving arginine-rich peptides? The number of arginines is around 4 and above, while the size of the peptide is...
10 July 2021 9,196 3 View
Hello I am just wondering if peptide analogues containing D amino acids are more hydrophilic than its L-amino acid-containing analogues in HPLC? I synthesized and folded an all L-peptide, and the...
26 June 2021 7,360 2 View
Hello I am currently searching for literature about amino acid propensities to exclusively form certain secondary structures regardless of what kind of peptide or protein do they form (e.g....
14 May 2021 9,834 2 View
Hello I am just wondering if you have ideas or if you can share review articles or literatures about insights/strategies/methods for structure-activity relationship study of peptides? I am only...
09 May 2021 4,047 3 View
I am also searching for circular dichroism databases that also have information for venom peptides. However, most of the databases available on the internet are only for proteins.
29 March 2021 8,725 1 View
Right now, I am using ProtParam to compute GRAVY index of peptides. However, some of my peptides are post-translationally modified, and contains unusual amino acids. I assume that ProtParam does...
30 January 2021 8,513 4 View
The cleavage cocktail is composed of Phenol/thioanisole/water/TFA/triisopropylsilane. I only prepare 10 mL cocktail per peptide but can the activity of the cocktail prepared at 100 mL last for a week?
11 January 2021 7,648 3 View
