28 Questions 26 Answers 0 Followers
Questions related from Simon Miguel Lopez
Hello I want to ask if you encountered papers that showed different behaviors when using similar opsins? For example, when you use archaeorhodopsin or halorhodopsin, did they produced different...
24 August 2023 3,399 0 View
Good Day I am currently reviewing concepts, and practicing using a epifluorescent microscopy. I often notice that there is a right shift in the histogram of my images. I would like to ask/seek...
19 August 2023 3,473 0 View
Hello Everyone I am using 0.25% tryspin to digest my cells for passage/seeding. However, I just notice that the cell quality is not good based on appearance (e.g. many rough cells and clusters...
23 May 2023 2,975 3 View
Good Day. I am doing patch clamp recordings on neurons and HEK cells. I would like to know what are the recommended sampling rates for neurons and HEK cells. I am concerned that my high sampling...
09 May 2023 4,993 3 View
Hell Everyone I am finding out a convincing reason by asking for your thoughts and opinions. I noticed there are are several cre-lines that can be used to express your protein in a brain region...
16 April 2023 1,948 1 View
Good Day Recently in my AP recordings, I often observe that there is a time delay in my current injection recordings. For example, if I set the protocol to inject at 1s, the actual recording...
19 March 2023 2,348 0 View
March 19, 2023 Hello I often observe a small -2.1 pA being injected into my cell, even if I already have a 0 pA holding current in my protocol. Do you also have a similar experience? What could...
19 March 2023 1,563 0 View
Hello I am using HEK293 as a model cell line to study a heteromeric receptor. Based from your experiences (including other cell lines that also studies heteromeric receptor). How many passages...
16 March 2023 9,399 0 View
Good Day. I am patching primary cortical neurons. I am just curious if you can predict based only on bright-field microscopy and neuron morphology, what neuron types are present in primary...
11 March 2023 597 0 View
Good Day. I am just curious if there is any reference that shows what age of primary cortical neurons have a membrane potential range from -60 to -70 mV? Right now, the membrane potential of my...
11 March 2023 5,134 0 View
Hello Is there a possibility that there is an uneven distribution of polylyine when coating a coverslip? How do you solve this? Hoping for your insightful answers. Thanks.
02 March 2023 3,982 0 View
Good Day Based from your experience, I would like to ask if it is usual that there are dead and floating transfected HEK293 cells after seeding in PLK-coated coverslip? In my experiments, I used...
30 January 2023 2,070 1 View
Hello everyone. Recently, I observe that my HEK293 cells in the 6 well plate have circular cells that are floating in the medium. This is only a small fraction of the whole cell maybe
28 January 2023 8,777 2 View
Good Day. I am doing patch-clamp electrophysiology of ligand-gated ion channels (LGIC) in HEK293 cells, and I am still working my way to understand the technical details of managing the perfusion...
27 January 2023 3,382 0 View
Hello I am planning to do a quality check of my antibodies to different GABAA receptor via immunocytochemistry? I have a1, b2 and gamma2s subunits. If I want to verify that the b2 antibody binds...
21 December 2022 1,123 3 View
Hello. I am just curious if there will be changes in function/behaviour if you optogenetically activate a particular region of the neuron (e.g. dendrite), compared to activating the whole neuron...
05 November 2022 6,300 2 View
Hello I am just wondering if anyone has an experience with touchdown PCR during their site-directed mutagenesis experiments. Based on my past conventional PCR experiments, I do not see my...
14 September 2022 7,676 0 View
Hello. I would like to ask if there is a rough criterion or ballpark figure when to say if the compound administered to the mouse is in high dose or low dose? For instance, if the compound is...
02 March 2022 6,686 0 View
Hello I just want to ask if you can also recommend research or review articles highlighting the unique receptors found on CD4+ T cells that are not found on other immune cells? Thank you
26 October 2021 2,414 1 View
Hello I just want to gather insights on how much time does your reaction take when cleaving arginine-rich peptides? The number of arginines is around 4 and above, while the size of the peptide is...
10 July 2021 9,261 3 View
Hello I am just wondering if peptide analogues containing D amino acids are more hydrophilic than its L-amino acid-containing analogues in HPLC? I synthesized and folded an all L-peptide, and the...
26 June 2021 7,420 2 View
Hello I am currently searching for literature about amino acid propensities to exclusively form certain secondary structures regardless of what kind of peptide or protein do they form (e.g....
14 May 2021 9,887 2 View
Hello I am just wondering if you have ideas or if you can share review articles or literatures about insights/strategies/methods for structure-activity relationship study of peptides? I am only...
09 May 2021 4,126 3 View
I am also searching for circular dichroism databases that also have information for venom peptides. However, most of the databases available on the internet are only for proteins.
29 March 2021 8,867 1 View
I am pondering on the idea if it is possible to couple an "N-terminal with Fmoc-protection (but unprotected side chain) & with PTM-modification (e.g. brominated & gamma carboxylated)"...
05 February 2021 1,762 1 View
Right now, I am using ProtParam to compute GRAVY index of peptides. However, some of my peptides are post-translationally modified, and contains unusual amino acids. I assume that ProtParam does...
30 January 2021 8,656 4 View
The cleavage cocktail is composed of Phenol/thioanisole/water/TFA/triisopropylsilane. I only prepare 10 mL cocktail per peptide but can the activity of the cocktail prepared at 100 mL last for a week?
11 January 2021 7,820 3 View
Hello everyone. I would like to ask for your insights/impression on what could be the therapeutic advantage of targeting ion channels in the brain or spinal cord? What could also be the advantage...
01 January 1970 5,699 1 View
