What are the importance of trizol in RNA extraction?

More Khetam Qaid Mayee's questions See All

What is the relationship between ladder and voltage in gel electrophoresis ?

ladder, gel electrophoresis, pcr

31 December 2017 6,127 3 View

How does a difference in GC content in primers affect PCR?

primer

31 December 2017 7,218 0 View

How to remove DNA contamination from extracted RNA?

TRIzol extraction, DNA contamination, RNA, Extraction

11 December 2017 5,493 5 View

What is the faster journals in publishing of genotyping and midecal research and within scopus?

Genotyping, Medical journals

05 June 2017 4,513 7 View

DNA extraction from formaldehyde preserved samples?

I have Jellyfish sample preserved in 4% formaldehyde, how could i extracte DNA ?? and What is the probability of getting enough DNA con.? DNA, Jellyfish, preserved samples

04 May 2017 8,175 4 View

How much proteinase K adding when extracting DNA from hard tick?

proteinase K, DNA, extraction, hard tick,

04 May 2017 5,335 3 View

What is the main viral zoonotic diseases circulating in the Arab World ?

virology, zoonotic diseases, RNA, DNA

04 May 2017 7,598 7 View

What are DNA Vaccines?

DNA

03 April 2017 2,371 6 View

I need program for phylogentic tree easily using more then MEGA6 ?

phylogentic tree, MEGA6

03 April 2017 4,667 8 View

What are the differences between extracted RNA and cDNA?

RNA, cDNA

03 April 2017 1,221 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View