What are the housekeeping genes that you usually use in the study of oral epithelial cell?

12 December 2013 1 229 Report

I'm doing my study on cytokines from epithelial cell prior infection of mixed biofilm Candida albicans. Therefore, I would like to know what is the best housekeeping gene if I want to study the expression of genes in oral epithelial cells.

Tim Maisch

You can use ß-actin, Pdi or GAPDH as housekeeping genes. We used for Candida an anti-GAPDH polyclonal antibody (a b9385, Abcam, Cambridge, UK)

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Science method

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