What kit did you use to extract RNA from mixed biofilms of yeast and bacteria?

09 September 2013 1 9K Report

My research is about mixed yeast-bacteria biofilm and I'm quite confused as to what kit I should use to isolate RNA from the mixed biofilm. I want to study the expression of both microbes with RT-PCR soon. What I know is, I can use any RNA extraction kit, but, the primer which I'm going to use in RT-PCR should be specific as to differentiate between yeast and bacteria. What you all guys think?

Thomas James Greer Wilson

The yeast mRNA will have a poly-A tail. Not so common in bacteria

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