Hello,
I am Mahmuda, now I do ExpiCHO-S cell culture. As suspension cells, these cell cultures are very susceptible to clumping. This clumping really made my cell culture unhealthy. Are there any tips to make ExpiCHO-S cell culture healthy? I hope my cell culture has better viability
Thank you for the help
Hello Mahmuda Mahmuda
Cell clumping is influenced by a variety of factors, including basal culture medium, media additives, culture vessels and agitation rates. Screening and selection of the right culture medium is a good way to reduce cell clumping. Adding anti-clumping agents such as Pluronic F-68 or Dextran sulfate to the culture can also help to alleviate clumping.
Please refer to the article attached below.
Article Combination of dextran sulfate and recombinant trypsin on ag...
Best.
Dear Mahmuda Mahmuda,
You can also try,to cultivate the cellline in spinnerflask. This can lower the chances of clumping.
If you are working with differentiated MSCs a lot you should check-out Cellseeker https://www.cellseeker.org/). This is an easy and free to use app to organize and inventory your cell collection online.
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