Hello,
I am Mahmuda, now I am working with DG44 cell culture. So far, my cell culture viability improved to 90%. However, before transfection, my cell viability decreased to 70%. I am very disappointed with the results of this culture.
Is there any suggestion or input that can be given so that I can solve this problem?
Then, is there any particular trick to do for this DG44 culture?
Thank you for the help
Saurabh Mandal
hi,
If you have used Lipofectamine then cell death is a very common problem. You need to serially dilute the Lipofectamine reagents & lower them down to that volume where you can see less toxicity and efficient transfection. Otherwise, if possible can think to switch off to another product.
Regards
Saurabh
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