Hi, I am interested in doing pluripotency analysis of my mESCs, and want to include the earliest markers for differentiation. I do observe some form of differentiation in my colonies, but not sure as to which lineage they could belong to. I am looking for something that's probably before the neural lineage, but ahead of naive pluripotent nanog/oct4 expressing stage?
Many thanks.
Has anyone used 'Murine 5T4
Oncofoetal Antigen' as differentiation onset marker? http://jcs.biologists.org/content/116/22/4533
NKX2.1 is an early marker of fore-brain tissue. We use that for lung and pancreas as well since that shows up immediately following ectoderm/endoderm induction
Dear Pooja,
I would suggest
Gata-4 (yolk sac endoderm and later cardiac marker) , GATA-6 extraembryonic endoderm and visceral endoderm
Sox1-neuroectoderm
T (Brachiury) - early mesoderm
loss of SSEA-1 and nanog as general sighs of differentiation
alpha-fetoprotein - endoderm
Fgf5 is a neural precursor marker that gets up-regulated pretty fast following differentiation. It can come earlier than a lot of canonical neuro-ecotdermal markers.
Hello
I agree with researchers here and you can see my published on RG which explain the differentiation markers of embryonic stem cells in this link
https://www.researchgate.net/publication/282441254_Specifics_references_in_Similarities_And_Differences_Between_Embryonic_And_Cancer_Stem_Cells
Also read the other links
Hope help you
Good Luck
http://jcs.biologists.org/content/116/22/4533.long
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3549628/
http://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.0020158
http://www.sabiosciences.com/rt_pcr_product/HTML/PAMM-081A.html
http://genesdev.cshlp.org/content/19/10/1129.long
Research Specifics references in Similarities And Differences Between...