Why does the value of displacement applied results in different Load-deflection curve behavior?

Hello, I am trying to model a four point bending for RCC beam using abaqus CDP model. I used displacement control type. The issue is whenever I change the value of the applied displacement, it...

25 September 2020 4,395 2 View

Is glucose sensor has another application or target rather than diabetes ?

I need a chapter or paper discussing list of application of glucose sensors , can anyone help ? thanks

02 June 2020 6,635 1 View

Hi, What do you think are new and practical issues for writing dissertations in the healthcare system?

What new and practical issues are important in the management and economics of the healthcare system in the world? Please provide your suggested titles on these topics. Thank you for answering...

10 January 2019 4,801 8 View

Hello, How can I measure the rotation angle from human face image in matlab?

I need to calculate the human face's rotation angle of face images like the following examples. For example, the first image has rotated 10 degrees to the right or the second image has rotated 90...

05 December 2018 3,112 5 View

How can one find the data for agricultural products such as production quantity, export, etc , in two categories: developing and developed countries?

These categories are not mentioned at the "special groups" of FAOSTAT.

19 October 2018 2,475 13 View

What is the best preservation of RNA extract?

I need to know the best way to preserve RNA extract from tissue for a long period.

27 October 2017 4,716 9 View

Dear researchers, By any chance do you know any center that has clinical trial in stem cell therapy for type 1diabetes?

Case is a 4 year old kid, recently diagnosed with type I diabetes, seeking for new opportunities and novel treatments. Beside, parents banked his/her cord blood stem cells. I appreciate if you...

07 May 2016 7,210 4 View

Do you know any studies or books about artificial intelligence and supply chain management ?

18 December 2015 6,601 4 View

How can I avoid high mortality of rotenone-induced PD?

I used 1.5mg/kg dissolved in DMSO/PEG 1:1 and I have high mortality intraperitoneal injection.

30 October 2014 4,152 1 View

How can I estimate the sequence of the SiRNA that will be used?

I want to prevent the expression of 11 amino acid in the peptide chain using siRNA,

16 February 2014 5,441 2 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View