What are the differentiation markers of vascular smooth muscle cells to osteoblast (mineralized SMCs) in flow cytometry?

Daniel Moreno Martinez @Daniel_Moreno_Martinez

11 November 2014 1 9K Report

I want to perform flow cytometry to measure differentiation of hVSMCs to osteoblast-like cells, but I am unsure of which commercial surface marker antibodies I should use.

Susanne Duncker

If you want to detect fully functional osteoblasts, you could use an antibody against OPG, the RANKL decoy receptor secreted by osteoblasts.

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Science method

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