Can I perform gene expression calculation without housekeeper gene in qPCR?

I do not have Ct values for housekeeper genes, as those may not be present in the sample and including them would be misleading. I need to be able to compare Ct values without housekeeping...

09 October 2015 3,477 5 View

Who can suggest a microparticle isolation method that doesn't involve isolation of the MPs together with the exosome fraction?

I want to isolate the microparticle fraction from blood samples. I have seen plenty of protocols and commercial products to isolate exosomes, but I am interested in microparticle isolation. As far...

02 March 2014 9,348 8 View

Which media suits HUVECs?

I am getting HUVECs from someone that does not know where they come from nor the media they were grown before. I have never worked with HUVECs and I have no idea which media could be used. Can I...

02 March 2014 1,748 16 View

Fluorochrome combination in a blue & red cytometer?

Is there any combination of fluorochromes, in a blue & red laser cytometer (FACSVerse), that allows me to do a 3 color setup with no overlapping or little? At the moment I am using FITC, PE and...

11 December 2013 5,276 1 View

How can I set up the flow analysis in a FACSVerse?

I am trying to analyze Endothelial Microparticles in a FACSVerse, however I am struggling to set up the cytometer. FYI: Threshold is 200 on FSC PMT: FCS 186, SSC 250, FITC 430, APC 476 & PE...

10 November 2013 6,006 2 View

BD FACSVerse common problems?

I would like to know if you have ever found any problems working with a FACSVerse? I have had the chance to work with two of them in different labs and I have always had problems with them....

10 November 2013 2,644 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View