I want to isolate the microparticle fraction from blood samples. I have seen plenty of protocols and commercial products to isolate exosomes, but I am interested in microparticle isolation. As far as I know, differential centrifugation will allow me to isolate MPs but together with the exosome fraction, which is not useful for me and may disturb any further analysis. Any ideas or suggestions?
How do you define "microparticle"? Exosomes are sometimes considered to be a subset of microparticles, rather than an entirely different entity.
Thank you Kenneth. I define microparticles as particles smaller than 1um but bigger than 0.1um, beign smaller than 0.1um particles the exosome fraction
I am interested in isolating the microparticles but without the exosome nor apoptotic bodies fraction.
What technique are you planning on using to analyse your microparticles?
Thank you very much Joshua.
My interest does not lay in their flow cytometric analysis. What I really want to do get is "pure" population of just microparticles. I am not sure whether it is possible to sort them using FACS or not.
Thank you very much for your suggestions, I will try them!
How do you think to use sucrose gradient which is usually used to isolate virus? As far as I know, sucrose gradient can be prepared using different density to isolate different size of exosome/microparticles.
Thank you Ming, I will take a look at sucrose gradients as well!
Hi Daniel,
did you manage to set up the method of microparticles' isolation?
Because I have the same problem you had - I would like to isolate microparticles (0.2-1um) from blood.
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