7 Questions 13 Answers 0 Followers
Questions related from Daniel Moreno Martinez
I do not have Ct values for housekeeper genes, as those may not be present in the sample and including them would be misleading. I need to be able to compare Ct values without housekeeping...
10 October 2015 3,547 5 View
I want to perform flow cytometry to measure differentiation of hVSMCs to osteoblast-like cells, but I am unsure of which commercial surface marker antibodies I should use.
11 November 2014 9,415 1 View
I want to isolate the microparticle fraction from blood samples. I have seen plenty of protocols and commercial products to isolate exosomes, but I am interested in microparticle isolation. As far...
03 March 2014 9,401 8 View
I am getting HUVECs from someone that does not know where they come from nor the media they were grown before. I have never worked with HUVECs and I have no idea which media could be used. Can I...
03 March 2014 1,801 16 View
Is there any combination of fluorochromes, in a blue & red laser cytometer (FACSVerse), that allows me to do a 3 color setup with no overlapping or little? At the moment I am using FITC, PE and...
12 December 2013 5,332 1 View
I am trying to analyze Endothelial Microparticles in a FACSVerse, however I am struggling to set up the cytometer. FYI: Threshold is 200 on FSC PMT: FCS 186, SSC 250, FITC 430, APC 476 & PE...
11 November 2013 6,060 2 View
I would like to know if you have ever found any problems working with a FACSVerse? I have had the chance to work with two of them in different labs and I have always had problems with them....
11 November 2013 2,714 2 View
