Recently, I have referred to many papers on establishing pancreatic cancer metastasis to the liver. Among them, I was curious to ask why most of the papers used non-orthotopic injection methods but chose injection methods such as iPO, IV, or IS.

What are the differences between intraportal (iPO) injection, intrasplenic (IS) injection, and intravenous (IV) injection in establishing a mouse model of pancreatic cancer metastasis to the liver?

The cells I am currently culturing include mouse pancreatic cancer cells and human pancreatic cancer cell lines. When I want to try injecting these cells into mice, I am not sure which method I should follow.

At the same time, I have another question: Can cancer cells with YFP fluorescence be grown in mice without being destroyed? If I culture these cells in vitro, do I need to avoid light stimulation during culture? , like turning off the lights.

Thank you in advance for your kind responses and help.