I already get SNP candidates for our target traits, then I would like use Real-time PCR based genotyping platform to check F1 population.
For establishing the marker, the SNP flanking sequences are needed.
Does any one know?
Thank you very much
https://genome.ucsc.edu/
Choose Genome Browser, then type your SNP in the search field and you'll get the 500bp sequence around it.
the additional information in the attached paper gives archives of whole genome sequence which may be BLAST searched.
http://prunusmumegenome.bjfu.edu.cn
Use samtools, faidx options
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