Hi Peterhs Hsiang,

the key enzymes of the aerobic glycolysis pathway are hexokinase, phosphofructokinase and pyruvate kinase.

Best,

Murat

Hello Peterhs Hsiang

Glucose metabolism in tumor is governed by both oncogenes and cancer-producing factors. Metabolic reprogramming is a complex interaction of various signaling pathways, such as Notch, AKT, phosphoinositide-3-kinase (PI3K), PTEN, etc.

You may select PTEN (Phosphatase and Tensin Homolog deleted on Chromosome 10) for Western Blot detection. PTEN not only plays a role in growth and survival but also in metabolic regulation. PTEN regulates signals in metabolic regulation which includes glycolysis, gluconeogenesis, glycogen synthesis, lipid metabolism as well as mitochondrial metabolism.

It is a dual phosphatase with both protein and lipid phosphatase activities. PTEN reduces the level of PI-3, 4, 5-P3 (PIP3), a critical second messenger mediating the signal of not only the growth factors but also that of insulin. Increase in PIP3 recruits AKT to the membrane where it is activated by other kinases also dependent on PIP3. Many components of this pathway have been described as causal forces in cancer. PTEN activity is lost by mutations, deletions, or promoter methylation silencing at high frequency in many primary and metastatic human cancers.

Best.

thanks for all information here