Trung Nghia

8 Questions 17 Answers 0 Followers

Questions related from Trung Nghia

What is cause of decrease in effectiveness in fermentation scaling up process?

Hi, I am working on Pichia pastoris fermentation. I had runned on 7.5 litter scale, small scale. I have yet tried at 25 litter scale, big scale. The result shows I got failure in scaleup. You can...

04 April 2019 3,478 10 View

What make the different in protein expression of the same carbon source?

Hi, I am working on protein expression by Pichia pastoris. I focus on mixed feed to get higher result. I read many papers. Some of author said that glycerol is repression the AOX1 promoter, which...

05 May 2018 3,216 0 View

Why every time the target protein get out of the gel filtration column, it made overpressure?

Dear, I am doing in gel filtration. My protein is fgf2. In the first step of purification, I applied cation exchange chromatography, but it was not clear enough. I try to do the next step to get...

07 July 2017 9,287 5 View

Which perfect buffer should I use in Bradford assay with many protein sample?

Hi, I'm purifying a recombinant protein. It's secreted protein. After chromatography procedure, I have some samples of protein: - Fermentation sample (applied sample): the supernatant from BSM...

10 October 2016 1,200 12 View

What are the differences between BSM and BMGY medium?

Hi, I'm studying in the fermentation of P. pastoris. I try to find out what difference between BSM and BMGY. I just know the elements of those media, but I don't know the role of each element....

07 July 2016 5,702 2 View

How much samples can I apply to a cation exchange column?

Hi, I'm using Hitrap SP FF collumn (cation exchange collums) to purifiy PDGF. The collumn's details show binding capacity of some proteins: 70 mg Ribonuclease A/ml gel (Mw: 13 700 D) 50 mg human...

04 April 2015 2,399 6 View

Can FPLC destroy protein structure to many oligopeptides which can be not detected by SDS-PAGE electrophoresis?

I purify by FPLC by Hitrap SP FF column. I have 4 fractions and all of them were being collected. I take an SDS-PAGE electrophoresis, and get some things below: - Percentage of target protein in...

01 January 2015 2,136 10 View

How can I fix unknown lines in SDS-PAGE electrophoresis?

Dear, I take a SDS-PAGE electrophoresis gel. It appears some lines which I don't know what they are. The line connect all wells of gel and they are more than two. What's it and How to fix my...

12 December 2014 2,820 16 View