I would like to know whether there are differences between 3D cells cultured in magnetic assisted well and mammospheres cultured in ultra low attachment well in terms of their characteristics and properties. 

What to observe and what to measure if I would like to treat mammospheres with a compound? Must I measure spheres sizes in time point or Centeral Zone diameter whether it become smaller or wider? When I read papers, I noted that using 3D for cytotoxicity test has not fully standardized yet. Then. I am confuse what to do with such situations and what are we going to show as results?