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Questions related from Mingchuan Yu
I am transfecting mammalian cells to express my target protein. In order to know if my transfection is successful, instead of WB, I would like to check with qPCR. However, cDNA sequence of my...
18 November 2022 6,706 0 View
I tried analysis of angiogenesis by usign imageJ. However, I noted that the imageJ does not make tracing of some tubes and network. The software usually tracked and traced those network of vessels...
01 March 2020 5,305 2 View
I used software to fit Sigmoid curve to get IC50 but the curve generated was not a typical Sigmoid shape. Why does it so? I have series of 10 different concentration of a compound and tested for...
16 February 2020 4,882 4 View
Can anyone share the cheapest price of WST dye brands? I am using now MA dye from Biolog and it is a kind of WST dye too.
28 August 2019 5,873 0 View
I would like to buy biologically active human VEGFA protein. Not sure where to get it? Appreciate if anyone can share links of supplier names for it. I found one below from abcam and I think it is...
25 July 2019 6,729 1 View
I have found out For Human, Monocytes, we can use CD14, CD16. For FACS sorting, we must have other abs of surface markers for white blood cells whole population CD45, To get rid of T cells we...
17 May 2019 3,875 2 View
I would like to do FACS sorting for mouse monocytes after ficoll separation. However, I am confused for information I get about those surface markers for mouse monocytes. Some listed about 18 abs...
17 May 2019 8,652 4 View
I noted many discussions about SD or SEM in qPCR relative gene expression study. Some recommended that we do not need to show SD as it does not make sense any in this analysis. Some added SD or...
27 November 2017 9,223 11 View
I was looking for ingredients of serum free freezing medium but I cannot find anywhere or its discussion in this research gate. So Can anyone share your knowledege on making serum free freezing...
02 October 2017 7,287 4 View
I like to know whether it is possible just to wash out trypsin with PBS, centrifuge followed by resuspending the pellet with serum free medium for cells to be cultured with serum free medium?
24 September 2017 8,193 11 View
This particular qPCR melting curve (refer the attached) has issue on its left side of the curve as it was a big higher. Ran 3 times with same qPCR profile and got 3 different melt curve as you see...
17 June 2017 3,772 6 View
I would like to know whether there are differences between 3D cells cultured in magnetic assisted well and mammospheres cultured in ultra low attachment well in terms of their characteristics and...
17 June 2017 6,163 1 View
I would like to know what happened to one melt curve that I get from qPCR. Among 6 products, only one this product showed such melt curve. I repeated again and also gave me the same one while...
10 April 2017 2,350 6 View
I would like to understand more about Phenotypic microarrays experiments by using PMM plates in order to find metabolomic pathways of mammalian cells. Kindly let me know if you are doing or you...
04 November 2016 1,275 0 View
I know cell cycle, how it works and we are looking for which step of cell cycle is affected after treating with a compound. This must be the reason for studying cell cycle analysis. What I don't...
05 August 2016 6,466 5 View
Does anyone experience in culturing human kidney proximal tubules epithelial cells lines (HK2 cell line) culture in Quantum 286. Is this medium Quantum 286 expensive compare to DMEM? Since this...
15 June 2016 7,087 0 View
From which supplier comapnies, Can we buy human kidney fibroblast cells such as TK173 , TK 455, TK188? I cannot find suppliers on internet. Do they adhere properly to cultural flasks and plates?...
09 June 2016 2,762 0 View
I would like to know more about staining cell culture wells infected with virus in TCID50 test. I noticed that some of them use methylene blue or trypan blue. Most of them use these stains for...
23 June 2015 8,594 8 View
