How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How do I show the data values on the Y-as of a boxplot?

Hi everyone! I have a question: I made a boxplot from a question in my survey asked with a 5-point Likert scale in SPSS. The question was 'Do you execute frequently a CT of the skull?" with...

07 November 2020 9,319 5 View

CCT software - brain lesion volume - surface?

I am planning to project a subcortical/cortical brain lesion volume onto the skull surface from a CCT data set. I would be grateful for hints for an appropriate & free software solution for...

17 March 2020 890 4 View

Anyone could give some tips for fish brain microdisection?

I want to isolate fish brain specific zones like telencephalon, cerebelum, opticum tectum. I am encoutering with the problem of hardness of tissue because in the moment we try to take fish brain...

04 May 2019 6,386 1 View

Anyone could give some tips for fish brain microdisection?

I want to isolate fish brain specific zones like telencephalon, cerebelum, opticum tectum. I am encoutering with the problem of hardness of tissue because in the moment we try to take fish brain...

30 April 2019 8,841 1 View

How to use Brain Extraction Tool(BET)?

Hi , I want to extract brain from other tissue and skull in MRI Images by BET(Brain Extraction Tool) , but I cant download it as software or use it as code, I cant find any type of these.

03 April 2019 4,942 3 View

To all Skull experts - how limited are conclusion drawn from 2D skull measurements?

Dear Skull experts, I have 2D measurements of turtle skulls and like to know how limited I am in my conclusions drawn from that. One aim of my study is to provide an identification key to the...

02 March 2019 7,286 8 View

What are the correct coordinates to target RTN nucleus?

To target retrotrapezoid nucleus (RTN) in adult Wistar rats for drug micro-injections via steel cannula what are the coordinates with respect from Bregma (AP, DV and ML). Different authors have...

31 December 2018 1,943 0 View

Mouse Cortrex L2\3 DV cordinate ?

I want to deliver viral vector to layer 2/3 in mouse cortex. What is the DV coordinate recommended and where the depth count start from ? is it the skull surface itself or the cortex surface?

20 December 2018 5,931 4 View

Mouse Cortrex L2\3 DV cordinate ?

I want to deliver viral vector to layer 2/3 in mouse cortex. What is the DV coordinate recommended and where the depth count start from ? is it the skull surface itself or the cortex surface?

11 December 2018 3,321 5 View

What is the typical distance from the surface of the skull to the brain in adult mice?

I am performing stereotaxic injection in mice. In the Paxinos Mouse Brain Atlas it appears that the depth is relative to the brain surface at bregma (z=0). The hole I drill in the skull is tiny,...

10 November 2018 5,327 5 View