Are there any inherent cell line model that is resistant to tamoxifen. ZR-75-1 is also a luminal cell line with ER an Her2 expression, but in real clinical condition they are not treated with tam but antiher2 and AI. so is it posible to compare MCF-7 as a sensitive model and ZR-75-1 as a resistnat model.
best,
charitha
You can check the dose response of Tamoxifen in both the cell lines and determine which cell line is more sensitive to Tamoxifen.
Dear Dr. Gangadharam,
One of the tests now available is the "CYP2D6" test, which manifests metabolism of tamoxifen in different host cells. Your MCF-7 and ZR-75-1 cells might be compared in that test. CYP2D6 is important to generating 4-hydroxy-N-desmethyl tamoxifen, which is the most abundant of the high-affinity 4-hydroxylated metabolites. So, you might comprehensively evaluate the evidence to date on the association between CYP2D6 inhibition and tamoxifen effectiveness through a review and meta-analysis of the literature regarding CYP2D6 and breast cancer outcomes.
Best wishes,
Ilya
Zr-75-1 cells are not a HER2+ cell line, although this cell line express higher level than MCF7. In published data, both MCF7 and ZR-75-1 cell lines have a similar IC50 for tamoxifen (sligthly higher for ZR-75-1). I would test a few ER+ lines it like Sandeep suggested.
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