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Questions related from Charitha Gangadharan
i am trying to look specific CAF markers. In this regards i found that Fibroblast specific protein (S100A4) is a CAF marker. But the primary characterisation papers suggest another protein...
21 October 2017 8,716 0 View
There are enzymes used for dissociation of cells. Gibco has variety of trypsin. it had .25% trypsin and trypsin with different con of EDTA. My query is when do we use trypsin and when to use...
07 September 2017 7,276 1 View
Could anybody please tell me what are the different types of cells that originate from HNSCC patient samples. What are the type of studies that it can be used for? I understand there are...
26 August 2016 514 2 View
I am trying to study drug induced apoptosis by flow cytometry. Is it necessary to collect the soup of cell culture media while trypsisnising and pelleting the cells after desired treatments. since...
17 May 2016 7,871 7 View
why does the resistant cells do not ahere to manually coated coverslips for IF. what is the suggested options other than using chamber slides?
29 February 2016 4,748 5 View
miRNAs are epigentic regulators and altered expression is reported in cancer. How to find out mutations in miRNA genes and their relevance in cancer? or are the mutaions in miRNA genes or its...
20 July 2015 4,836 2 View
Are there any inherent cell line model that is resistant to tamoxifen. ZR-75-1 is also a luminal cell line with ER an Her2 expression, but in real clinical condition they are not treated with tam...
10 June 2015 5,436 3 View
Has anybody looked at the allred score of MCF-7 and T47D cell lines? I understand that one is PR positive and the other negative. What is the status of expression of ER relatively in these two...
02 May 2015 4,932 1 View
Can we use a lentiviral (promoter/ expression) construct for transfection in MCF7 cells by lipofectamine based method? If not how can I modify the construct for an antibiotic based selection...
11 April 2015 1,912 10 View
