What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

How to simulate 5G Quality of Service characteristics?

For my research, I would like to simulate an end to end 5G network. My goal is to observe the behaviour of SDAP protocol, dynamic configuration of 5QI values etc. To summarize I would like to...

21 February 2021 7,624 3 View

SSR primers are species specific or we can use it for any plant species ?

Hi, any one please guide me; SSR primers are species specific or trait specific. i have few primer which was reported in articles for wheat yield can i use these similar primer for lentil yield...

20 February 2021 315 4 View

How is Initial GDP measured ?

Dear All, I am working on estimating Debt-Growth Threshold but confused about some points. My questions are; - How do we calculate initial GDP Per Capita? Shall we do it in STATA or excel file?...

15 February 2021 2,677 2 View

How to get actual or true SoC while predicting SoC for Batteries using Data Driven Approaches?

I have seen it in multiple research articles that authors use certain approaches to predict electric vehicle battery's state of charge(SoC) and then they compare it with battery's true SoC. So I...

11 February 2021 9,070 3 View

How fishery by-products/fish waste can be utilised as efficiently, cost-effectively and eco-friendly?

Fishery by-products utilisation in the industry with minimum cost and low carbon emissions.

29 December 2020 1,752 11 View

Techniques of Machine/Deep Learning for Battery's SoC Estimation?

Hi everyone I want to know which techniques of Machine Learning/Deep Learning I can use for more accurate SOC estimation of Lithium Ion batteries. Thanks and Regards

24 December 2020 2,096 1 View

How can I know which sequence is the correct one if there are lot of predicted sequence in database for my target gene?

Right now, I want to design qPCR primer for my target gene. But that gene has 21 predicted mRNA sequence in the NCBI database. Most of the sequence names are same but only different location...

23 December 2020 5,531 12 View

How to rectify the miscomputation of Pressure and Continuity in HVAC CFD Simulation Using OpenFOAM?

I'm using OpenFOAM to run a CFD study of thermal comfort within a hall. I'm using OpenFOAM v1912 and BouyantBoussinesq(Simple&Pimple)Foam solver. eI have 6 inlets and outlets each with specific...

21 December 2020 1,303 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Will it be possible to get IC:50 if we target cancer cell line with antibody drug conjugate (ADC) that have 50% less antigen positive cells ?

Is it possible to attain IC50 if my target cells have less than 50% antigen expression ? For example: Cancer cell line XXX is showing 35% of expression of cell surface antigen YYYY, if i...

04 February 2021 2,711 3 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

IC50 value from this graph?

01 January 2021 6,848 4 View

I am performing enzyme inhibition assay and the substrate is fluorogenic & fluorescence was measured. Now I want to calculate Vo and Vi. How can I ??

These are the per minute fluorescence observed. Please guide me how can I determine Vi, Vo and IC50 ? By slope determination or any other method? Control: 28.11 40.30 40.78 37.61...

11 December 2020 1,037 4 View

How to determine non-cytotoxic drug concentration using MTT assay?

Many literature showed that how to determine IC50 drug concentration using MTT assay. But I want to know how to determine non-cytotoxic concentration of a particular drug with maximum its...

31 October 2020 2,012 3 View

How do I find the IC50 and best drug treatment time for anticancer drug?

I want to find the IC50 of a drug on a cancer cell line. I'm not sure how long the treatment time should be for in vitro experiment. Is there an assay/protocol I can follow to determine the IC50...

15 October 2020 8,678 6 View

Are you using pIC50 instead of IC50?

Hi, An IC50 is the concentration at which a ligand (drug, extract, ...) can inhibit an enzyme by 50%. pIC50 is a new approach to examine the same data in a logarithmic manner. I ask the RG...

24 September 2020 1,531 3 View

IC50 for plant extracts?

If I am working with plant extracts and their photoprotective properties.... It is just enough to evaluate the citotoxicity at a specific (high) concentration of my extracts or it is necessary to...

14 September 2020 8,890 1 View

What is the correct standard IC50 limit for a crude plant extract in cancer cell ?

i want a stander ic50 for plant to know about the strong of my crud extract plant in cancer cell line

24 July 2020 2,461 2 View