My team is currently studying bacterial biofilms, but have had trouble getting our strain of E. coli to form strong biofilms. We're currently using E. coli 25922 (I believe) growing in M9 media. We're using 24 and 96 well flat bottom plates. Plates are tissue culture treated non-pyrogenic polystyrene.
After some deliberation with my team and research professor we've decided to try a new strain of E. coli for our experiment. I was hoping to get some input about what strains are superior biofilm formers?
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I know about E. coli strain O157:H7, E. coli strain O26, E.coli strain K-12, most enterohemorrhagic and enteropathogenic strains of E. coli will form biofilms
Stain K12 is very much pathogenic but you can use other strains to do that
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