What are the best solvents to precipitate Aromatic PolyAmic Acid from its solution in DMF or DMAC?

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Biomerieux API Campy performance?

Hello, I have a somewhat weird question: has anyone had problems with campylobacter growing in the second half of the strip GLU through ERO? Have you managed to correct it? There is some...

19 July 2024 3,094 2 View

Which edition do you recommend?

which publication do you recommend for submitting an article on the philosophical and sociological analysis of AI vs Society

30 January 2024 3,918 2 View

Can I add my preprints posted in the Zenodo repository to my list of scientific papers?

preprints on various scientific topics

04 July 2023 5,965 3 View

Could someone suggest a company working in the field of artificial meat (cellular agriculture)?

Hi, I am looking for a company operating in the field of artificial meat. By operating, I mean production or advanced stage of pre-production. I am looking for because we have some good solutions...

24 November 2022 7,258 8 View

Could anyone suggest a source of water soluble oligomers of polylactide?

I have seen in the literature that short chain poly-lactic acid (polylactide; PLA) oligomers are water soluble. But I am unable to find a commercial source of this type of oligomers. Does anyone...

31 October 2022 1,419 6 View

Can anyone suggest a mercury-free LED light source for fluorescence microscopy?

Can anyone suggest a mercury-free LED light source for fluorescence microscopy? I'm looking for the simplest and most economical replacement for mercury bulbs. Not the complicated stuff with many...

05 October 2022 6,057 3 View

WST-1, Roche, Cytotoxicity calculation?

Please only answer if you have done this and do not send me paper links. Thanks. Hi technical Gurus :) So, I am working on a cytotoxicity assay based on the Roche's WST-1 reagent. Every setup is...

15 September 2022 1,611 0 View

Could isopropyl alcohol (2-propanol) be used instead of DMSO for the stock solutions for cell cultures?

For various reasons, I need to avoid DMSO with my cells. So I wonder if isopropyl alcohol (2-propanol) could be used instead of DMSO to create my stock solutions library? I have learned that the...

04 July 2022 3,655 1 View

Why is the most common used liposome composition is DOPC/DOPG (80/20)?

Based on the literature analysis it could be concluded that one of the most common composition of synthetic lipids to prepare liposome mixture or model membrane (particularly in the work with...

28 June 2022 9,817 3 View

What are the possible reasons for fibroblastic transformation of confluent CHO-K1?

Hello to all I observed a strange phenomenon with CHO-K1 following the change of serum batch (it is surely the toxicity of this batch but I am very curious about the possible nature of this...

21 April 2022 6,157 3 View

What is the solvent for 8YSZ nano material?

I need to do some experiment with it in liquid form.

07 August 2024 613 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

29 July 2024 4,570 0 View

My cfDNA extraction protocol using silica beads works with plasma collected in COD SAGM bags but not with plasma collected in K2 EDTA tubes. Why so?

I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...

28 July 2024 3,559 0 View

What is the Principle of Cysteine-carbazole-sulfuric acid reaction with glucose?

I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...

23 July 2024 3,891 0 View

Why methanol and sulphuric acid used in the analysis of polyhydroxyalkanoates (PHA) by GC-MS?

Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?

22 July 2024 1,210 2 View