How to calculate Leaf Water Conten Index (LWCI)?

I want to calculate Leaf Water Conten Index (LWCI) using Landsat or Sentinel data. I found several usefull formulas that use NIR and SWIR bands. But to estimate LWCI I have to have NIR and SWIR...

16 March 2020 9,322 4 View

Real Time PCR Analysis and Normalization?

If I measure gene expression in an organism under two different conditions, do I still need to normalize these genes to a housekeeping gene or comparison between the genes under two different...

18 December 2018 6,267 2 View

Gene Expression Analysis using R?

Hello fellow researchers, Can anyone recommend a package for R for gene expression analysis using R?

13 December 2018 5,140 5 View

Monoclonal antibody purity?

If monoclonal antibody would be obtained from ascites fluid during hybridoma expression in vivo -> protein A/G-sepharose purification, how much non-specific AB contamination and non-target...

04 December 2018 6,582 3 View

Does the plasmonic near field spread on the distance sufficient for the resonance light absortion (scattering)?

Cross section of light absorption and light scattering for noble metal (silver, gold) nanoparticle is higher than its geometrical cross section in case of plasmon resonance. It can be...

30 October 2018 5,235 3 View

Degenerate Primers to ITS Region (16S-23S rRNA) for NGS?

F: 50 -CTKTTGGGYYCCCKGRYYG-30 Rev: 50-CGCGTCCACTMTCCAGTTCTC-30 These are primers for NGS sequencing of bifidobacteria from a publication. If you substitute, for example K, with G or T then...

09 April 2018 6,351 3 View

How to obtain a precipitated powder of Poly-Amic Acid based on aromatic diamine and aromatic dianhydride?

How to obtain a precipitated powder of Poly-Amic Acid (PAA) based on aromatic diamine and aromatic dianhydride? It is well known that for several Aromatic Polyimides (PIs) in the course of...

22 December 2017 9,399 3 View

Which solvent or a combination of solvents could you propose for controlled swelling of Cellophane film ?

Which solvent or a combination of solvents could you propose for controlled swelling of Cellophane film

17 August 2017 1,331 1 View

I need dataset (training set) for image stegoanalysis on a base of neural networks. Are there such bases? Does anybody know?

I need dataset (training set) for image stegoanalysis on a base of neural networks. Are there such bases? Does anybody know? I know about stegoDB but can't find download link. Thanks

10 April 2017 8,150 9 View

Is that C. jejuni?

Can someone take a look and confirm whether what I isolated is indeed C. jejuni? I haven't got any primers yet, so I can't confirm by PCR, but the culture is gram negative, catalaze +, oxidase +....

26 March 2017 3,470 12 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View