What are the best programs and methods to computationally dock RNA onto proteins?

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What is the best way to make antibodies against DNA and DNA-RNA hybrids?

We are examining structures of non-B-DNA such as DNA or DNA-RNA G quadruplex and would like to test if these occur in cells using antibodies. I know some G guadruplex antobides exist but we are...

08 September 2018 6,384 7 View

What are rigorous assays to measure the impact of a given enzyme and its inctivation on mutation rates, patterns, and signatures?

I am intersted in measuring the impact of specific enzymes and their inactivating mutations on genome instability and on the mutation burden in human cells and cancers. I want experiment ways to...

06 July 2018 2,257 0 View

Are scientific meetings a net expense that should be limited or do they save research time and money?

Many institutions are limiting travel and meeting attendance with the idea of saving money. Yet, I think meetings always save research money for me. I find that when I attend meetings the...

10 November 2017 2,132 4 View

What are the best ways to detect dsDNA in the cytoplasm?

re there specific stains and fluorescent methods to detect dsDNA in the cytoplasm of cells? Are these quantitative? What are the best controls and methods to validate dsDNA in cytoplasm?

09 October 2017 5,217 1 View

Given the issues with journal impact factors, would it be helpful to have some type of author-based metrics or impact factors or not?

Since the use of journal impact factors as frequently used to evaluate researh quality has been criticised for many reaons, might it be useful to devise mertics based upon a given author's impact....

11 December 2016 1,925 3 View

'What methods have you found to be effective for increasing your lab's productivity?

07 August 2016 6,071 2 View

What are the best ways to cross-link proteins to DNA?

07 August 2016 1,452 2 View

How can we best teach students to answer questions where they have not learned the answer?

We largely teach students and then ask them questions that we taught them how to answer. In science and many other areas of life, one wishes to address and answer questions where you have not...

06 July 2016 6,509 2 View

What do you see as the major contributions that will come from cry-electron miscopy in the next decade?

11 December 2015 8,615 0 View

What are good ways to efficiently measure methane binding?

We are working on enzyme engineering to convert methane to alcohols. As part of this process we wish to measure methane binding to various enzymes and would benefit from suggestions as to what has...

31 December 2014 9,321 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Avinash S. Punekar

Dear John,

I have used HADDOCK (http://www.bonvinlab.org/software/haddock2.2/) - information-driven felxible docking approach for modelling protein-protein, protein-nucleic acid and protein-ligand complexes. The results include additional scripts for further analysis and scoring, which is very helpful.

Best wishes,

Avinash