What do you see as the major contributions that will come from cry-electron miscopy in the next decade?

12 December 2015 0 9K Report

Are there breakthroughs in structural, mechanistic, and predictive knowledge of macromolecules expected form cro-EM that are likley to major impacts on synthetic biology, medicine, and the bioeconomy?

Badges
Science topic

Similar topics
Medicine

More John Tainer's questions See All

What are the best programs and methods to computationally dock RNA onto proteins?

We have used DOT to dock DNA to proteins but increasingly need to examine computational models for RNA docking onto proteins. Would appreciate advice and examples where this has proven successful.

10 November 2019 7,982 4 View

What is the best way to make antibodies against DNA and DNA-RNA hybrids?

We are examining structures of non-B-DNA such as DNA or DNA-RNA G quadruplex and would like to test if these occur in cells using antibodies. I know some G guadruplex antobides exist but we are...

08 September 2018 6,519 7 View

What are rigorous assays to measure the impact of a given enzyme and its inctivation on mutation rates, patterns, and signatures?

I am intersted in measuring the impact of specific enzymes and their inactivating mutations on genome instability and on the mutation burden in human cells and cancers. I want experiment ways to...

06 July 2018 2,416 0 View

Are scientific meetings a net expense that should be limited or do they save research time and money?

Many institutions are limiting travel and meeting attendance with the idea of saving money. Yet, I think meetings always save research money for me. I find that when I attend meetings the...

10 November 2017 2,284 4 View

What are the best ways to detect dsDNA in the cytoplasm?

re there specific stains and fluorescent methods to detect dsDNA in the cytoplasm of cells? Are these quantitative? What are the best controls and methods to validate dsDNA in cytoplasm?

09 October 2017 5,379 1 View

Given the issues with journal impact factors, would it be helpful to have some type of author-based metrics or impact factors or not?

Since the use of journal impact factors as frequently used to evaluate researh quality has been criticised for many reaons, might it be useful to devise mertics based upon a given author's impact....

11 December 2016 2,084 3 View

'What methods have you found to be effective for increasing your lab's productivity?

With decreased funding for scientific research there is a compelling need to make the most efficient progress possible without sacrificing rigor or impact. I would value hearing what you have...

07 August 2016 6,219 2 View

What are the best ways to cross-link proteins to DNA?

I have a protein that binds DNA bubbles and that I think is a dimer on DNA. I do not know if one or two subunits bind to DNA. I would like to cross-link subunits adjacent to DNA (bound) to see...

07 August 2016 1,607 2 View

How can we best teach students to answer questions where they have not learned the answer?

We largely teach students and then ask them questions that we taught them how to answer. In science and many other areas of life, one wishes to address and answer questions where you have not...

06 July 2016 6,678 2 View

What are good ways to efficiently measure methane binding?

We are working on enzyme engineering to convert methane to alcohols. As part of this process we wish to measure methane binding to various enzymes and would benefit from suggestions as to what has...

31 December 2014 9,449 4 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View

In SEM, if a latent variable consists of three observed variables, and each observed variable has four items, what should I do?

Suppose I am studying the construct of "Academic Motivation" (latent variable) in students, which is measured through three observed variables: "Intrinsic Motivation," "Extrinsic Motivation," and...

31 July 2024 3,913 6 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Bootstrapping in SEM Amos ?

Can a researcher use small sample size of 40 while using SEM with bootstrapping of 1000 and is it possible to get published

28 July 2024 4,402 2 View

Do you know of any online international conferences that offer free discussions?

Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.

28 July 2024 6,501 0 View