My aim is to develop and enhance my cloning to make expression in my victor which contains promoters and Tumor gene i got difficulty how i get start
I thought which course can make me more understanding and interpret complex in cancer research area before i start any phD program
10 November 2018 2,249 0 View
Dear All ... I hope this finds you well, I am from Ireland, I have got MSc in cancer biology and i would like to study PhD somewhere, I would be grateful if I could study under your...
09 October 2018 5,851 0 View
this is first time i do cell culture in the Lab and i have wondering how i can do that ? where i should start ? which manual could i use ? please help me out
31 December 2017 5,335 1 View
I have read a paper about plasmid and PCR then i found this Tm
31 December 2017 5,741 2 View
Writing a good review article is a real challenge could anyone tell me how to do that
11 December 2017 8,823 2 View
i tried to understand some theoretical biochemistry concepts while i am working with the some basic Lab Technics could you please help me out
10 November 2017 6,011 6 View
I am going to do my first restriction digest in the next few days with unequal sites , cut will be in two parts , linear plasmid
10 November 2017 853 0 View
clone gene with BglII and EcoRI flanking into puc19
09 October 2017 2,577 4 View
If you don't have that experience or teacher to help you what should to do
09 October 2017 915 0 View
just i asking how i do that theoretically
09 October 2017 7,876 0 View
I have selected brain tumor images ...but now found that already lots of research done n this topic.
03 March 2021 5,774 3 View
Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...
03 March 2021 401 4 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?
01 March 2021 8,087 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...
28 February 2021 5,440 3 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...
25 February 2021 3,221 7 View