What are the best cloning strategies to insert cancer gene with different promoters perform expression?

03 March 2017 0 423 Report

My aim is to develop and enhance my cloning to make expression in my victor which contains promoters and Tumor gene i got difficulty how i get start

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More Osama el Ghwail's questions See All

How I become supper Interpretation in science, genetics in cancer research?

I thought which course can make me more understanding and interpret complex in cancer research area before i start any phD program

10 November 2018 2,249 0 View

I am looking for applying PhD position in cancer biology ?

Dear All ... I hope this finds you well, I am from Ireland, I have got MSc in cancer biology and i would like to study PhD somewhere, I would be grateful if I could study under your...

09 October 2018 5,851 0 View

Any suggestion on which cell culture handbook is the best ?

this is first time i do cell culture in the Lab and i have wondering how i can do that ? where i should start ? which manual could i use ? please help me out

31 December 2017 5,335 1 View

Does anybody known this word meaning Tm ?

I have read a paper about plasmid and PCR then i found this Tm

31 December 2017 5,741 2 View

Any suggestions on how to write good biochemistry literature review ?

Writing a good review article is a real challenge could anyone tell me how to do that

11 December 2017 8,823 2 View

Is there any theoretical handbook about basic Lab tech such as minikit ,transformation and so on ?

i tried to understand some theoretical biochemistry concepts while i am working with the some basic Lab Technics could you please help me out

10 November 2017 6,011 6 View

How do i perform Restriction digestion Pmax cloning vector ?

I am going to do my first restriction digest in the next few days with unequal sites , cut will be in two parts , linear plasmid

10 November 2017 853 0 View

How i do clone with two restriction enzymes BglII and EcoRI into PUc19 plasmid ?

clone gene with BglII and EcoRI flanking into puc19

09 October 2017 2,577 4 View

Have a guidelines and some knowledge but you do not have any exprience in the Lab even your time is not enough to submit your project ?

If you don't have that experience or teacher to help you what should to do

09 October 2017 915 0 View

How I Clone Gene with Hind III and Spe I restriction sides flanking into pUC19 MCS ?

just i asking how i do that theoretically

09 October 2017 7,876 0 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View