What will be the possible reasons to cause no bands on gel electrophoresis?

Hi, I am doing RNA extraction for my final year project. After extraction of RNA by using conventional method, I did quantification and qualification of RNA by using Nanodrop spec and gel...

11 January 2021 8,775 10 View

How to analyze Longitudinal Pre/Post different subjects?

I have 6 years of pre program data for two schools and I have 6 years of Post program data for the same two schools. The issue is that the POST data is not in the same years and the PRE data. I'm...

05 January 2021 4,587 5 View

Independent T tests a priori with MANOVA?

I am conducting a causal-comparative study for my dissertation, and am under quality review. The reviewer told me that I should prepare for post hoc testing by conducting independent t tests a...

16 October 2019 5,465 3 View

HEK293 Cell line detachment after being adhered for over 3 days?

08 December 2016 9,523 2 View

Any advice on why my Her2 protein is not being coated to my amine plate?

I coat 1 ug/mL of the Her2 protein with 100 uL 100mM carbonate buffer to my flat bottomed well and have only had a positive result once out of 8 times. I have tried coating at 4 deg C overnight,...

30 November 2016 3,720 5 View

How can criminalizing prostitution and sex trade aid in healthcare intervention for such victimization and abuse?

07 July 2016 4,742 2 View

Are human B and T cells adherent or suspension cells in culture? I think they're sticking to the bottom of my plate. Possible?

26 April 2016 2,726 6 View

Does anyone have any interesting references on memory distortion and eyewitness evidence?

02 April 2015 1,285 4 View

Any suggestions to get the characteristic red pigment expressed by Microsporum gallinae?

Microsporum gallinae ATCC 90749; received strain from ATCC and have grown on Sabouraud dextrose agar at various temperatures.

03 April 2014 249 2 View

Problem with Touchdown PCR for DRD2 SNP.

I'm doing analysis on a DRD2 SNP for ADHD patients, with Touchdown PCR and electrophoresis. For the last 3 weeks I had to work on another PCR machine and now the Touchdown PCR doesn't work at all...

07 February 2013 6,119 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Hello house, is it necessary to compute Content Validity Ratio (CVR) for an adapted questionnaire pls?

What should one do if he obtains conflicting results between Content Validity Index (CVI) and CVR for items in a quetionnare. For example based on the CVI obtained the items are acceptable but the...

25 February 2021 7,325 2 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View