Our study is about to isolate nanocellulose crystals from leaves and to do that we are finalizing our methods for the prelab which will be the isolation of cellulose.
Many researchers in the field opt for mesh sizes in the range of 20 to 60 mesh (0.84 mm to 0.25 mm), as this range has been found to facilitate effective maceration of plant tissues while minimizing damage to the cellulose fibers. Please see the attached link for complete details. https://www.google.com/url?sa=t&source=web&rct=j&opi=89978449&url=http://hdjaincollege.org/fileupload/uploads/60bb27209f5a920210605072624B.Sc%2520Botany%2520Part%2520II%2520Hons%2520Paper%2520Practical%2520Maceration%2520techniques.pdf&ved=2ahUKEwib8-Hws_CAAxVgbvUHHXuVAUwQFnoECBYQAQ&usg=AOvVaw3KLIub2ON1BnzKlzBAnfTB
The recommended mesh size for sample preparation in a study related to the isolation of cellulose from leaves is 50 micrometers (μm). This mesh size is large enough to allow the cellulose fibers to pass through, but small enough to remove any large particles that could interfere with the isolation process.
A study published in the journal Bioresource Technology in 2017 found that using a 50 μm mesh size resulted in the highest yield of cellulose from leaves. The study also found that using a smaller mesh size (25 μm) resulted in a lower yield of cellulose, as the smaller mesh size blocked the passage of some of the cellulose fibers.
Here are some other factors to consider when choosing a mesh size for sample preparation in the isolation of cellulose from leaves:
The type of leaves you are using. Some leaves, such as hardwood leaves, have thicker cell walls than other leaves, such as softwood leaves. This means that you may need to use a larger mesh size for hardwood leaves to allow the cellulose fibers to pass through.
The concentration of the alkaline solution. A higher concentration of alkaline solution will make the cellulose fibers more soluble, which means that you may be able to use a smaller mesh size.
The amount of time that the leaves are extracted in the alkaline solution. A longer extraction time will allow more of the cellulose to be extracted, which means that you may be able to use a smaller mesh size. (cite properly if you use any references in given answer) Thanx🙏🙏🙏
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