I am trying the synthesis of graphene oxide by using modified hummer's method and the color of my product was white silver so is it graphene oxide?

i have tried modified hummer's method for the synthesis of graphene oxide by adding Graphite powder and NaNO3 in H2SO4 . In this mix KMnO4 was added and diluted with water . this mix was heated...

13 January 2021 9,423 4 View

Is direct contact of liquid nitrogen to tissue/cells an issue?

I have un-replaceable tissues to process for several experimentation such as MS/ WB/ IHC etc. However, I suspect the direct contact of liquid nitrogen to my samples for the duration of few...

18 October 2020 9,375 3 View

How XRD intensity is affected with density of powders ?

I mixed 60 vol% boron carbide (B4C), 25 vol% titanium diboride (TiB2), 10 vol% silicon carbide (SiC) and 5 vol% alumina (Al2O3) powders in jar mill for 12 hours using toluene as mixing...

17 June 2020 1,433 13 View

How can i solve a set of 3 equations having one of the element as non linear involving sigmaudot of the response u?

I need to use matlab to get the displacement and velocity time history of the 3 degree of freedom system (image attached). i can use any numerical method or ode45 but one of the element in third...

25 September 2019 3,820 5 View

How can I apply a thermo-mechanical load in hypermesh?

I want to determine the stresses for a laminated composite plate under thermo-mechanical load.

24 May 2019 9,180 4 View

Can I get MATLAB code for vibrational analysis of composite plate?

Iam working on vibrational analysis of laminated composite plate using MATLAB.

28 October 2018 7,641 3 View

Required information regarding impact testing machine for polymer composites?

Hello guys, i am working on characterisation and design application of polymer composites. I want to perform the pendulum impact testing of the fabricated composite, for this i need fracture...

24 March 2018 7,143 3 View

Why xrd data of powder materials do not have stable baseline throughout 2theta range(10-90) . Baseline shifts from top to down as angle increases?

why xrd data of powder materials do not have stable baseline throughout 2theta range(10-90) . Baseline shifts from top to down as angle increases?

18 April 2017 4,844 4 View

How can I extract triazole from DMSO solvent?

05 December 2014 8,498 3 View

Why am I not getting structures in synthesizing of bimetallic core-nanoshell nanoparticles?

I need some help synthesizing bimetallic core-nanoshell nanoparticle of Ru:Pd:Ni. Why am I not getting such structures? Can anybody help me out?

05 May 2014 2,528 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View